Artículo
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
Lucero Estrada, Cecilia Stella Marys
; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia
; Stefanini de Guzman, Ana Maria Teresa Valentina
Fecha de publicación:
06/2007
Editorial:
Elsevier Science
Revista:
Food Research International
ISSN:
0963-9969
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora.
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Articulos(CCT - SAN LUIS)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Citación
Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina; Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR; Elsevier Science; Food Research International; 40; 5; 6-2007; 637-642
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