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dc.contributor.author
Scarlet, Dragos
dc.contributor.author
Handschuh, Stephan
dc.contributor.author
Reichart, Ursula
dc.contributor.author
Podico, Giorgia
dc.contributor.author
Ellerbrock, Robyn E.
dc.contributor.author
Demyda Peyrás, Sebastián
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dc.contributor.author
Canisso, Igor F.
dc.contributor.author
Walter, Ingrid
dc.contributor.author
Aurich, Christine
dc.date.available
2022-03-17T21:06:57Z
dc.date.issued
2021-08
dc.identifier.citation
Scarlet, Dragos; Handschuh, Stephan; Reichart, Ursula; Podico, Giorgia; Ellerbrock, Robyn E.; et al.; Sexual differentiation and primordial germ cell distribution in the early horse fetus; Multidisciplinary Digital Publishing Institute; Animals; 11; 8; 8-2021; 1-18
dc.identifier.issn
2076-2615
dc.identifier.uri
http://hdl.handle.net/11336/153544
dc.description.abstract
It was the aim of this study to characterize the development of the gonads and genital ducts in the equine fetus around the time of sexual differentiation. This included the identification and localization of the primordial germ cell population. Equine fetuses between 45 and 60 days of gestation were evaluated using a combination of micro-computed tomography scanning, immunohistochemistry, and multiplex immunofluorescence. Fetal gonads increased in size 23-fold from 45 to 60 days of gestation, and an even greater increase was observed in the metanephros volume. Signs of mesonephros atrophy were detected during this time. Tubular structures of the fetal testes were present from day 50 onwards, whereas cell clusters dominated in the fetal ovary. The genital ducts were well-differentiated and presented a lumen in all samples. No sign of mesonephric or paramesonephric duct degeneration was detected. Expression of AMH was strong in the fetal testes but absent in ovaries. Irrespective of sex, primordial germ cells selectively expressed LIN28. Migration of primordial germ cells from the mesonephros to the gonad was detected at 45 days, but not at 60 days of development. Their number and distribution within the gonad were influenced (p < 0.05) by fetal sex. Most primordial germ cells (86.8 ± 3.2% in females and 84.6 ± 4.7% in males) were characterized as pluripotent according to co-localization with CD117. However, only a very small percentage of primordial germ cells were proliferating (7.5 ± 1.7% in females and 3.2 ± 1.2% in males) based on co-localization with Ki67. It can be concluded that gonadal sexual differentiation in the horse occurs asynchronously with regard to sex but already before 45 days of gestation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Multidisciplinary Digital Publishing Institute
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
FETUS
dc.subject
GENITAL DUCT
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GERM CELL
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GONAD
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HORSE
dc.subject.classification
Tecnología GM, clonación de ganado, selección asistida, diagnósticos, tecnología de producción de biomasa, etc.
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dc.subject.classification
Biotecnología Agropecuaria
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Sexual differentiation and primordial germ cell distribution in the early horse fetus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-03-14T21:06:00Z
dc.journal.volume
11
dc.journal.number
8
dc.journal.pagination
1-18
dc.journal.pais
Suiza
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dc.journal.ciudad
Basilea
dc.description.fil
Fil: Scarlet, Dragos. University of Veterinary Medicine Vienna; Suiza. Universitat Zurich; Suiza
dc.description.fil
Fil: Handschuh, Stephan. University of Veterinary Medicine Vienna; Suiza
dc.description.fil
Fil: Reichart, Ursula. University of Veterinary Medicine Vienna; Suiza
dc.description.fil
Fil: Podico, Giorgia. University of Illinois. Urbana - Champaign; Estados Unidos
dc.description.fil
Fil: Ellerbrock, Robyn E.. University of Illinois. Urbana - Champaign; Estados Unidos
dc.description.fil
Fil: Demyda Peyrás, Sebastián. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.description.fil
Fil: Canisso, Igor F.. University of Illinois. Urbana - Champaign; Estados Unidos
dc.description.fil
Fil: Walter, Ingrid. University of Veterinary Medicine Vienna; Suiza
dc.description.fil
Fil: Aurich, Christine. University of Veterinary Medicine Vienna; Suiza
dc.journal.title
Animals
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2615/11/8/2422
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ani11082422
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