Artículo
Sexual differentiation and primordial germ cell distribution in the early horse fetus
Scarlet, Dragos; Handschuh, Stephan; Reichart, Ursula; Podico, Giorgia; Ellerbrock, Robyn E.; Demyda Peyrás, Sebastián
; Canisso, Igor F.; Walter, Ingrid; Aurich, Christine
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
08/2021
Editorial:
Multidisciplinary Digital Publishing Institute
Revista:
Animals
ISSN:
2076-2615
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
It was the aim of this study to characterize the development of the gonads and genital ducts in the equine fetus around the time of sexual differentiation. This included the identification and localization of the primordial germ cell population. Equine fetuses between 45 and 60 days of gestation were evaluated using a combination of micro-computed tomography scanning, immunohistochemistry, and multiplex immunofluorescence. Fetal gonads increased in size 23-fold from 45 to 60 days of gestation, and an even greater increase was observed in the metanephros volume. Signs of mesonephros atrophy were detected during this time. Tubular structures of the fetal testes were present from day 50 onwards, whereas cell clusters dominated in the fetal ovary. The genital ducts were well-differentiated and presented a lumen in all samples. No sign of mesonephric or paramesonephric duct degeneration was detected. Expression of AMH was strong in the fetal testes but absent in ovaries. Irrespective of sex, primordial germ cells selectively expressed LIN28. Migration of primordial germ cells from the mesonephros to the gonad was detected at 45 days, but not at 60 days of development. Their number and distribution within the gonad were influenced (p < 0.05) by fetal sex. Most primordial germ cells (86.8 ± 3.2% in females and 84.6 ± 4.7% in males) were characterized as pluripotent according to co-localization with CD117. However, only a very small percentage of primordial germ cells were proliferating (7.5 ± 1.7% in females and 3.2 ± 1.2% in males) based on co-localization with Ki67. It can be concluded that gonadal sexual differentiation in the horse occurs asynchronously with regard to sex but already before 45 days of gestation.
Palabras clave:
FETUS
,
GENITAL DUCT
,
GERM CELL
,
GONAD
,
HORSE
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Licencia
Identificadores
Colecciones
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Scarlet, Dragos; Handschuh, Stephan; Reichart, Ursula; Podico, Giorgia; Ellerbrock, Robyn E.; et al.; Sexual differentiation and primordial germ cell distribution in the early horse fetus; Multidisciplinary Digital Publishing Institute; Animals; 11; 8; 8-2021; 1-18
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