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dc.contributor.author
Lescano López, Ignacio
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dc.date.available
2021-11-15T19:54:57Z
dc.date.issued
2020-06
dc.identifier.citation
Lescano López, Ignacio; Determination of ureides content in plant tissues; Bio-protocol LLC; Bio-protocol; 10; 11; 6-2020; 1-9; e3642
dc.identifier.issn
2331-8325
dc.identifier.uri
http://hdl.handle.net/11336/146919
dc.description.abstract
The ureides allantoin and allantoate are the main organic nitrogen compounds transported in several legumes, predominantly from N2 fixation. Moreover, recent studies point out a remarkable role for allantoin during several stress responses of plants other than legumes. The goal of this protocol is to determine ureides concentration in different plant tissues. Ureides are extracted from plant material by boiling it in phosphate buffer. The allantoin and allantoate present in the supernatants are subjected to alkaline-acidic hydrolysis to glyoxylate. The glyoxylate is converted into glycoxylic acid phenylhydrazone, that is then oxidized to red-colored 1,5-diphenylformazan. The absorbance of supernatants is measured using a spectrophotometer at 520 nm. Ureides concentration can be inferred by using a glyoxylate calibration curve. Ureide quantification of different tissues of Arabidopsis thaliana and soybean plants were carried out following this protocol.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Bio-protocol LLC
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ALLANTOIN
dc.subject
ALLANTOATE
dc.subject
UREIDES
dc.subject
PURINE
dc.subject
PLANTS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Determination of ureides content in plant tissues
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T15:14:29Z
dc.identifier.eissn
2331-8325
dc.journal.volume
10
dc.journal.number
11
dc.journal.pagination
1-9; e3642
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Sunnyvale
dc.description.fil
Fil: Lescano López, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.journal.title
Bio-protocol
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e3642
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.21769/BioProtoc.3642
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