Artículo
Determination of ureides content in plant tissues
Fecha de publicación:
06/2020
Editorial:
Bio-protocol LLC
Revista:
Bio-protocol
ISSN:
2331-8325
e-ISSN:
2331-8325
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The ureides allantoin and allantoate are the main organic nitrogen compounds transported in several legumes, predominantly from N2 fixation. Moreover, recent studies point out a remarkable role for allantoin during several stress responses of plants other than legumes. The goal of this protocol is to determine ureides concentration in different plant tissues. Ureides are extracted from plant material by boiling it in phosphate buffer. The allantoin and allantoate present in the supernatants are subjected to alkaline-acidic hydrolysis to glyoxylate. The glyoxylate is converted into glycoxylic acid phenylhydrazone, that is then oxidized to red-colored 1,5-diphenylformazan. The absorbance of supernatants is measured using a spectrophotometer at 520 nm. Ureides concentration can be inferred by using a glyoxylate calibration curve. Ureide quantification of different tissues of Arabidopsis thaliana and soybean plants were carried out following this protocol.
Palabras clave:
ALLANTOIN
,
ALLANTOATE
,
UREIDES
,
PURINE
,
PLANTS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Lescano López, Ignacio; Determination of ureides content in plant tissues; Bio-protocol LLC; Bio-protocol; 10; 11; 6-2020; 1-9; e3642
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