Artículo
HGF induces protective effects in α-naphthylisothiocyanate-induced intrahepatic cholestasis by counteracting oxidative stress
Salas Silva, Soraya; Simoni Nieves, Arturo; Razori, María Valeria
; López Ramirez, Jocelyn; Barrera Chimal, Jonatan; Lazzarini, Roberto; Bello, Oscar; Souza, Verónica; Miranda Labra, Roxana U.; Gutiérrez Ruiz, María Concepción; Gomez Quiroz, Luis Enrique; Roma, Marcelo Gabriel
; Bucio Ortiz, Leticia
Fecha de publicación:
04/2020
Editorial:
Pergamon-Elsevier Science Ltd
Revista:
Biochemical Pharmacology
ISSN:
0006-2952
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cholestasis is a clinical syndrome common to a large number of hepatopathies, in which either bile production or its transit through the biliary tract is impaired due to functional or obstructive causes; the consequent intracellular retention of toxic biliary constituents generates parenchyma damage, largely via oxidative stress-mediated mechanisms. Hepatocyte growth factor (HGF) and its receptor c-Met represent one of the main systems for liver repair damage and defense against hepatotoxic factors, leading to an antioxidant and repair response. In this study, we evaluated the capability of HGF to counteract the damage caused by the model cholestatic agent, α-naphthyl isothiocyanate (ANIT). HGF had clear anti-cholestatic effects, as apparent from the improvement in both bile flow and liver function test. Histology examination revealed a significant reduction of injured areas. HGF also preserved the tight-junctional structure. These anticholestatic effects were associated with the induction of basolateral efflux ABC transporters, which facilitates extrusion of toxic biliary compounds and its further alternative depuration via urine. The biliary epithelium seems to have been also preserved, as suggested by normalization in serum GGT levels, CFTR expression and cholangyocyte primary cilium structure our results clearly show for the first time that HGF protects the liver from a cholestatic injury.
Palabras clave:
ANIT
,
CHOLESTASIS
,
HGF
,
LIVER
,
OXIDATIVE STRESS
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Colecciones
Articulos(IFISE)
Articulos de INST.DE FISIOLOGIA EXPERIMENTAL (I)
Articulos de INST.DE FISIOLOGIA EXPERIMENTAL (I)
Citación
Salas Silva, Soraya; Simoni Nieves, Arturo; Razori, María Valeria; López Ramirez, Jocelyn; Barrera Chimal, Jonatan; et al.; HGF induces protective effects in α-naphthylisothiocyanate-induced intrahepatic cholestasis by counteracting oxidative stress; Pergamon-Elsevier Science Ltd; Biochemical Pharmacology; 174; 4-2020; 1-13
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