Artículo
Production, physicochemical characterization, and anticancer activity of methotrexate-loaded phytic acid-chitosan nanoparticles on HT-29 human colon adenocarcinoma cells
Ciro, Yhor; Rojas, John; Di Virgilio, Ana Laura
; Alhajj, Maria J.; Carabali, Gustavo A.; Salamanca, Constain H.
Fecha de publicación:
01/09/2020
Editorial:
Elsevier
Revista:
Carbohydrate Polymers
ISSN:
0144-8617
e-ISSN:
1879-1344
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Methotrexate-loaded phytic acid-chitosan nanoparticles were synthesized by ionic gelation assisted by high-intensity sonication. The nanoparticles were characterized by particle size, polydispersity index, zeta potential (ZP) and encapsulation efficiency. Their physical stability was evaluated at 4 °C and 40 °C, whereas the in-vitro methotrexate release was assessed at pH 7.4. The data were heuristically fit to first-order, Higuchi, Peppas-Sahlin and Korsmeyer-Peppas models of release kinetics. Anticancer activity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay on HT-29 human colon adenocarcinoma cells. Physicochemical analysis showed that the nanoparticles presented positive ZP values, sizes less than <300 nm and low polydispersity, except for systems formed with low amplitude sonication. The nanoparticles exhibited an adequate physical stability and a capability to modify methotrexate release by a non-Fickian mechanism, resulting in a more pronounced cytotoxic effect than the free drug on HT-29 human colon adenocarcinoma cells.
Palabras clave:
ANTICANCER
,
CHITOSAN
,
IONIC GELATION
,
METHOTREXATE
,
NANOPARTICLES
,
PHYTIC ACID
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CEQUINOR)
Articulos de CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Articulos de CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Citación
Ciro, Yhor; Rojas, John; Di Virgilio, Ana Laura; Alhajj, Maria J.; Carabali, Gustavo A.; et al.; Production, physicochemical characterization, and anticancer activity of methotrexate-loaded phytic acid-chitosan nanoparticles on HT-29 human colon adenocarcinoma cells; Elsevier; Carbohydrate Polymers; 243; 01-9-2020; 1-9
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