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dc.contributor.author
Vallarino, José G.
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Kubiszewski Jakubiak, Szymon
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Ruf, Stephanie
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Rößner, Margit
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Timm, Stefan
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Bauwe, Hermann
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Carrari, Fernando Oscar
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Rentsch, Doris
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Bock, Ralph
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Sweetlove, Lee J.
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Fernie, Alisdair R.
dc.date.available
2021-10-01T23:29:41Z
dc.date.issued
2020-12
dc.identifier.citation
Vallarino, José G.; Kubiszewski Jakubiak, Szymon; Ruf, Stephanie; Rößner, Margit; Timm, Stefan; et al.; Multi-gene metabolic engineering of tomato plants results in increased fruit yield up to 23%; Nature Research; Scientific Reports; 10; 1; 12-2020; 1-18
dc.identifier.issn
2045-2322
dc.identifier.uri
http://hdl.handle.net/11336/142320
dc.description.abstract
The capacity to assimilate carbon and nitrogen, to transport the resultant sugars and amino acids to sink tissues, and to convert the incoming sugars and amino acids into storage compounds in the sink tissues, are key determinants of crop yield. Given that all of these processes have the potential to co-limit growth, multiple genetic interventions in source and sink tissues, plus transport processes may be necessary to reach the full yield potential of a crop. We used biolistic combinatorial co-transformation (up to 20 transgenes) for increasing C and N flows with the purpose of increasing tomato fruit yield. We observed an increased fruit yield of up to 23%. To better explore the reconfiguration of metabolic networks in these transformants, we generated a dataset encompassing physiological parameters, gene expression and metabolite profiling on plants grown under glasshouse or polytunnel conditions. A Sparse Partial Least Squares regression model was able to explain the combination of genes that contributed to increased fruit yield. This combinatorial study of multiple transgenes targeting primary metabolism thus offers opportunities to probe the genetic basis of metabolic and phenotypic variation, providing insight into the difficulties in choosing the correct combination of targets for engineering increased fruit yield.
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application/pdf
dc.language.iso
eng
dc.publisher
Nature Research
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Multi‑gene
dc.subject
Metabolic engineering
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Tomato plants
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Multi-gene metabolic engineering of tomato plants results in increased fruit yield up to 23%
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-28T21:03:38Z
dc.journal.volume
10
dc.journal.number
1
dc.journal.pagination
1-18
dc.journal.pais
Reino Unido
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dc.description.fil
Fil: Vallarino, José G.. Institut Max Planck fur Molekulare Physiologie; Alemania
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Fil: Kubiszewski Jakubiak, Szymon. Institut Max Planck fur Molekulare Physiologie; Alemania
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Fil: Ruf, Stephanie. Institut Max Planck fur Molekulare Physiologie; Alemania
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Fil: Rößner, Margit. Institut Max Planck fur Molekulare Physiologie; Alemania
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Fil: Timm, Stefan. Universität Rostock; Alemania
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Fil: Bauwe, Hermann. Universität Rostock; Alemania
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Fil: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
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Fil: Rentsch, Doris. University of Bern; Suiza
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Fil: Bock, Ralph. Institut Max Planck fur Molekulare Physiologie; Alemania
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Fil: Sweetlove, Lee J.. University of Oxford; Reino Unido
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Fil: Fernie, Alisdair R.. Institut Max Planck fur Molekulare Physiologie; Alemania
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41598-020-73709-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-020-73709-6
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