Artículo
Evaluation of in vitro Antifungal Activity of Xylosma prockia (Turcz.) Turcz. (Salicaceae) Leaves Against Cryptococcus spp.
Folly, Mariany L. C.; Ferreira, Gabriella F.; Salvador, Maiara R.; Sathler, Ana A.; da Silva, Guilherme F.; Santos, Joice Castelo Branco; Santos, Julliana R. A. dos; Nunes Neto, Wallace Ribeiro; Rodrigues, João Francisco Silva; Fernandes, Elizabeth Soares; da Silva, Luís Cláudio Nascimento; de Freitas, Gustavo José Cota; Denadai, Ângelo M.; Rodrigues, Ivanildes V.; Mendonça, Leonardo M.; Monteiro, Andrea Souza; Santos, Daniel Assis; Cabrera, Gabriela Myriam
; Siless, Gastón Ezequiel
; Lang, Karen L.
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
02/2020
Editorial:
Frontiers Media
Revista:
Frontiers in Microbiology
ISSN:
1664-302X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cryptococcus species are responsible for important systemic mycosis and are estimated to cause millions of new cases annually. The available therapy is limited due to the high toxicity and the increasing rates of yeast resistance to antifungal drugs. Popularly known as “sucará,” Xylosma prockia (Turcz.) Turcz. (Salicaceae) is a native plant from Brazil with little information on its pharmacological potential. In this work, we evaluated in vitro anticryptococcal effects of the leaf ethanolic extract of X. prockia and its fractions against Cryptococcus gattii and Cryptococcus neoformans. We also evaluated phenotypic alterations caused by ethyl acetate fraction (EAF) (chosen according to its biological results). The liquid chromatography–mass spectrometry (LC-MS) analysis of EAF demonstrated the presence of phenolic metabolites that belong to three structurally related groups as majority compounds: caffeoylquinic acid, coumaroyl-glucoside, and caffeoyl-glucoside/deoxyhexosyl-caffeoyl glucoside derivatives. The minimum inhibitory concentration (MIC) values against C. gattii and C. neoformans ranged from 8 to 64 mg/L and from 0.5 to 8 mg/L, for ethanolic extract and EAF, respectively. The EAF triggered an oxidative burst and promoted lipid peroxidation. EAF also induced a reduction of ergosterol content in the pathogen cell membrane. These effects were not associated with alterations in the cell surface charge or in the thermodynamic fingerprint of the molecular interaction between EAF and the yeasts evaluated. Cytotoxic experiments with peripheral blood mononuclear cells (PBMCs) demonstrated that EAF was more selective for yeasts than was PBMCs. The results may provide evidence that X. prockia leaf extract might indeed be a potential source of antifungal agents.
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Colecciones
Articulos(UMYMFOR)
Articulos de UNID.MICROANAL.Y MET.FISICOS EN QUIM.ORG.(I)
Articulos de UNID.MICROANAL.Y MET.FISICOS EN QUIM.ORG.(I)
Citación
Folly, Mariany L. C.; Ferreira, Gabriella F.; Salvador, Maiara R.; Sathler, Ana A.; da Silva, Guilherme F.; et al.; Evaluation of in vitro Antifungal Activity of Xylosma prockia (Turcz.) Turcz. (Salicaceae) Leaves Against Cryptococcus spp.; Frontiers Media; Frontiers in Microbiology; 10; 2-2020
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