Artículo
Derivation of Intermediate Pluripotent Stem Cells Amenable to Primordial Germ Cell Specification
Yu, Leqian; Wei, Yulei; Sun, Hai Xi; Mahdi, Ahmed K.; Pinzon Arteaga, Carlos A.; Sakurai, Masahiro; Schmitz, Daniel A.; Zheng, Canbin; Ballard, Emily D.; Li, Jie; Tanaka, Noriko; Kohara, Aoi; Okamura, Daiji; Mutto, Adrián Angel
; Gu, Ying; Ross, Pablo J.; Wu, Jun
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
03/2021
Editorial:
Cell Press
Revista:
Cell Stem Cell
ISSN:
1934-5909
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. To date, however, no known PSCs have demonstrated dual competency for chimera formation and direct responsiveness to primordial germ cell (PGC) specification, a unique functional feature of formative pluripotency. Here, by modulating fibroblast growth factor (FGF), transforming growth factor β (TGF-β), and WNT pathways, we derived PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for direct PGC-like cell induction in vitro and are capable of contributing to intra- or inter-species chimeras in vivo. XPSCs represent a pluripotency state between naive and primed pluripotency and harbor molecular, cellular, and phenotypic features characteristic of formative pluripotency. XPSCs open new avenues for studying mammalian pluripotency and dissecting the molecular mechanisms governing PGC specification. Our method may be broadly applicable for the derivation of analogous stem cells from other mammalian species. Yu, Wu, and colleagues report the derivation of intermediate PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for direct PGC-like cell induction in vitro and capable of contributing to intra- or inter-species chimeras in vivo. XPSCs harbor molecular, cellular, and phenotypic features characteristic of formative pluripotency.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (IIBIO)
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Citación
Yu, Leqian; Wei, Yulei; Sun, Hai Xi; Mahdi, Ahmed K.; Pinzon Arteaga, Carlos A.; et al.; Derivation of Intermediate Pluripotent Stem Cells Amenable to Primordial Germ Cell Specification; Cell Press; Cell Stem Cell; 28; 3; 3-2021; 550-567.e12
Compartir
Altmétricas
Items relacionados
Mostrando titulos relacionados por título, autor y tema.
-
Solari, Claudia María ; Losino, Noelia Ivana ; Luzzani, Carlos Daniel ; Waisman, Ariel ; Bluguermann, Carolina ; Questa, María ; Sevlever, Gustavo; Miriuka, Santiago Gabriel ; Barañao, Jose Lino Salvador ; Guberman, Alejandra Sonia (Academic Press Inc Elsevier Science, 2011-07)
-
Oliveira, L. M. A.; Falomir Lockhart, Lisandro Jorge ; Botelho, M. G.; Lin, K. H.; Wales, P.; Koch, J. C.; Gerhardt, Elizabeth; Taschenberger, H.; Outeiro, T. F.; Lingor, P.; Schüele, B.; Arndt Jovin, D. J.; Jovin, T. M. (Nature Publishing Group, 2015-11)
-
Fisch, Samantha C.; Gimeno, Maria Laura ; Phan, Julia D.; Simerman, Ariel A.; Dumesic, Daniel A.; Perone, Marcelo Javier ; Chazenbalk, Gregorio D. (BioMed Central, 2017-10-18)