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dc.contributor.author
Bagatolli, Luis Alberto
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dc.contributor.author
Sanchez, Susana A.
dc.contributor.author
Hazlett, Theodore
dc.contributor.author
Gratton, Enrico
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dc.date.available
2021-09-06T20:25:08Z
dc.date.issued
2003-03
dc.identifier.citation
Bagatolli, Luis Alberto; Sanchez, Susana A.; Hazlett, Theodore; Gratton, Enrico; Giant vesicles, laurdan, and two-photon fluorescence microscopy: Evidence of lipid lateral separation in bilayers; Elsevier Academic Press Inc.; Methods In Enzymology; 360; 3-2003; 481-500
dc.identifier.issn
0076-6879
dc.identifier.uri
http://hdl.handle.net/11336/139750
dc.description.abstract
This chapter describes giant vesicles, Laurdan, and two-photon fluorescence microscopy. The combination of Laurdan, giant unilamellar vesicles (GUVs), and two-photon fluorescence microscopy has been extremely useful in producing a microscopic picture of lipid-phase coexistence in the GUV bilayer model system. Laurdan is a unique probe, giving simultaneous information about morphology and phase state of lipid domains from fluorescence images. The critical issues in membrane biophysics today are centered on the molecular dynamics of the bilayer structure. The interplay between lipids that results in the formation of domains on a bilayer surface and the interactions among these domains and relevant membrane-associated biomolecules is of particular interest. The advantages of using a microscope as the optical arrangement are clear. The light collection efficiency of a well-designed microscope is greatly enhanced over other optical arrangements. In addition, the flexibility of fluorescence microscopes creates for the spectroscopist a malleable optical compartment that can be designed and readily redesigned as needed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Academic Press Inc.
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Laurdan
dc.subject
GUVs
dc.subject.classification
Biofísica
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Giant vesicles, laurdan, and two-photon fluorescence microscopy: Evidence of lipid lateral separation in bilayers
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-08-30T14:39:16Z
dc.journal.volume
360
dc.journal.pagination
481-500
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Burlington
dc.description.fil
Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil
Fil: Sanchez, Susana A.. No especifíca;
dc.description.fil
Fil: Hazlett, Theodore. No especifíca;
dc.description.fil
Fil: Gratton, Enrico. No especifíca;
dc.journal.title
Methods In Enzymology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0076687903601242
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/S0076-6879(03)60124-2
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