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Artículo

Development of a third-generation biosensor to determine sterigmatocystin mycotoxin: An early warning system to detect aflatoxin B1

Díaz Nieto, César HoracioIcon ; Granero, Adrian MarceloIcon ; Garcia, D.; Nesci, Andrea VerónicaIcon ; Barros, Germán GustavoIcon ; Zon, María AliciaIcon ; Fernández, H.
Fecha de publicación: 03/2019
Editorial: Elsevier Science
Revista: Talanta
ISSN: 0039-9140
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Físico-Química, Ciencia de los Polímeros, Electroquímica

Resumen

A third-generation enzymatic biosensor was developed to quantify sterigmatocystin (STEH). It was based on a glassy carbon electrode modified with a composite of the soybean peroxidase enzyme (SPE) and chemically reduced graphene oxide. The optimal conditions to construct the biosensor were obtained through an experimental design based on the response surfaces methodology. The experiments were performed in 0.1 mol L−1 phosphate buffer solution, pH 5. Amperometric measurements were carried out at − 0.09 V vs Ag/AgCl (3 mol L−1 NaCl). The biosensor showed a lineal response in the concentration range from 6.9 × 10−9 to 5.0 × 10−7 mol L−1. The limit of detection was 2.3 × 10−9 mol L−1 for a signal: noise ratio of 3: 1. Values of the apparent Michaellis-Menten constant, KM app, obtained by using both Lineweaver-Burk and Eadi-Hofstee methods were (1.5 ± 0.2) × 10−6 and (1.2 ± 0.2) × 10−6 mol L−1, respectively. STEH was analyzed in corn samples spiked with STEH, with an average recovery of 96.5%. The biosensor was also used to determine STEH in corn samples inoculated with the Aspergillus flavus fungus, which is an aflatoxins producer. Considering that STEH is a precursor of aflatoxin B1 (AFB1) in its biological transformation, its decrease over time was related to the production of AFB1. The STEH concentration determined using the biosensor was in very good agreement with that determined by HPLC.
Palabras clave: AFLATOXIN B1 , BIOSENSOR , CHEMICALLY REDUCED GRAPHENE OXIDE , SOYBEAN PEROXIDASE ENZYME , STERIGMATOCYSTIN
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/138662
URL: https://linkinghub.elsevier.com/retrieve/pii/S0039914018310683
DOI: http://dx.doi.org/10.1016/j.talanta.2018.10.032
Colecciones
Articulos (IDAS)
Articulos de INSTITUTO PARA EL DESARROLLO AGROINDUSTRIAL Y DE LA SALUD
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(CCT - SALTA-JUJUY)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SALTA-JUJUY
Citación
Díaz Nieto, César Horacio; Granero, Adrian Marcelo; Garcia, D.; Nesci, Andrea Verónica; Barros, Germán Gustavo; et al.; Development of a third-generation biosensor to determine sterigmatocystin mycotoxin: An early warning system to detect aflatoxin B1; Elsevier Science; Talanta; 194; 3-2019; 253-258
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