Artículo
Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
Fecha de publicación:
08/2015
Editorial:
Elsevier Science
Revista:
International Journal Of Biological Macromolecules
ISSN:
0141-8130
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The formation of insoluble complexes between proteins and oppositely charged polyelectrolytes was assessed. Two pancreatic enzymes: trypsin and chymotrypsin, and two anionic synthetic polyelectrolytes: polyacrylate and polyvinylsulfonate, were used for the study at the pH range between 3.00 and 5.00. Two different titration curve shapes, representing two insoluble complexes formation mechanisms, were found. The turbidity of enzyme–polyelectrolyte mixtures is related to the increase either in the size or in the quantity of the insoluble complexes. Ionic strength destabilized insoluble complex formation. Finally, the kinetics of the process of insoluble complex formation at different conditions was studied.
Palabras clave:
Pe
,
Polyelectrolytes
,
Paa
,
Polyacrylate
,
Pvs
,
Polyvinilsulfonate
,
Tryp
,
Trypsin
,
Chtrp
,
Chymotrypsin
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos(IPROBYQ)
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Citación
Lombardi, Julia; Picó, Guillermo Alfredo; Boeris, Valeria; Physicochemical study of the formation of complexes between pancreatic proteases and polyanions; Elsevier Science; International Journal Of Biological Macromolecules; 79; 8-2015; 160-166
Compartir
Altmétricas