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Artículo

Gene cloning and heterologous synthesis of a haloalkaliphilic extracellular protease of Natrialba magadii (Nep)

de Castro, Rosana EstherIcon ; Ruiz, Diego MarioIcon ; Gimenez, Maria InesIcon ; Silveyra, María XimenaIcon ; Paggi, Roberto Alejandro; Maupin Furlow, Julie A.
Fecha de publicación: 09/2008
Editorial: Springer Tokyo
Revista: Extremophiles
ISSN: 1431-0651
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

The gene encoding the protease Nep secreted by the haloalkaliphilic archaeon Natrialba magadii was cloned and sequenced. Upstream of the nep gene, a region related to haloarchaeal TATA-box and BRE-like consensus sequences was identified. The nep-encoded polypeptide had a molecular mass of 56.4-kDa, a pI of 3.77 and included a 121-amino acid propeptide not present in the mature Nep. A Tat motif (GRRSVL) was also identified at residues 10 to 15 suggesting it is a substrate of the Tat pathway. The primary sequence of Nep was closely related to serine proteases of the subtilisin family from archaea and bacteria (50 to 85% similarity). The nep gene was expressed in Escherichia coli and Haloferax volcanii resulting in production of active Nep protease. In contrast to the recombinant E. coli strains in which Nep activity was only detected in cell lysate, high levels of Nep protein and activity were detected in the culture medium of stationary phase recombinant Hfx. volcanii strains. The Hfx. volcanii synthesized protease was active in high salt, high pH and high DMSO. This study provides the first molecular characterization of a halolysin-like protease from alkaliphilic haloarchaea and is the first description of a recombinant system that facilitates high-level secretion of a haloarchaeal protease.
Palabras clave: GENE CLONING AND EXPRESSION , HALOALKALIPHILIC PROTEASE , NATRIALBA MAGADII , SOLVENT TOLERANCE , TAT PATHWAY
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/133979
URL: https://link.springer.com/article/10.1007%2Fs00792-008-0174-6
DOI: http://dx.doi.org/10.1007/s00792-008-0174-6
Colecciones
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
de Castro, Rosana Esther; Ruiz, Diego Mario; Gimenez, Maria Ines; Silveyra, María Ximena; Paggi, Roberto Alejandro; et al.; Gene cloning and heterologous synthesis of a haloalkaliphilic extracellular protease of Natrialba magadii (Nep); Springer Tokyo; Extremophiles; 12; 5; 9-2008; 677-687
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