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dc.contributor.author
Asención Diez, Matías Damián
dc.contributor.author
Aleanzi, Mabel C.
dc.contributor.author
Iglesias, Alberto Alvaro
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Ballicora, Miguel A.
dc.date.available
2017-02-17T20:01:57Z
dc.date.issued
2014-07
dc.identifier.citation
Asención Diez, Matías Damián; Aleanzi, Mabel C.; Iglesias, Alberto Alvaro; Ballicora, Miguel A.; A Novel Dual Allosteric Activation Mechanism of Escherichia coli ADP-glucose Pyrophosphorylase: The role of pyruvate; Public Library Of Science; Plos One; 9; 8; 7-2014; e103888
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/13167
dc.description.abstract
Fructose-1,6-bisphosphate activates ADP-glucose pyrophosphorylase and the synthesis of glycogen in Escherichia coli. Here, we show that although pyruvate is a weak activator by itself, it synergically enhances the fructose-1,6-bisphosphate activation. They increase the enzyme affinity for each other, and the combination increases Vmax, substrate apparent affinity, and decreases AMP inhibition. Our results indicate that there are two distinct interacting allosteric sites for activation. Hence, pyruvate modulates E. coli glycogen metabolism by orchestrating a functional network of allosteric regulators. We postulate that this novel dual activator mechanism increases the evolvability of ADP-glucose pyrophosphorylase and its related metabolic control.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Allosteric Regulation
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Bacteria Glycogen
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Adp-Glucose Synthesis
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Synergic Regulation
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A Novel Dual Allosteric Activation Mechanism of Escherichia coli ADP-glucose Pyrophosphorylase: The role of pyruvate
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-23T20:14:11Z
dc.journal.volume
9
dc.journal.number
8
dc.journal.pagination
e103888
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Loyola University Chicago. Department of Chemistry; Estados Unidos
dc.description.fil
Fil: Aleanzi, Mabel C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
dc.description.fil
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
dc.description.fil
Fil: Ballicora, Miguel A.. Loyola University Chicago. Department of Chemistry; Estados Unidos
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0103888
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0103888


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