Artículo
Co-occurrence of clinically relevant β-lactamases and MCR-1 encoding genes in Escherichia coli from companion animals in Argentina
Rumi, María Valeria; Mas, Javier; Elena, Alan Xavier
; Cerdeira, Louise; Muñoz, Maria E.; Lincopan, Nilton; Gentilini, Élida R.; Di Conza, José Alejandro
; Gutkind, Gabriel Osvaldo
Fecha de publicación:
03/2019
Editorial:
Elsevier Science
Revista:
Veterinary Microbiology
ISSN:
0378-1135
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Extended-spectrum β-lactamase (ESBL), plasmid-mediated AmpC (pAmpC) and MCR-1 phosphoethanolamine transferase enzymes have been pointed out as the main plasmid-mediated mechanisms of resistance to third generation cephalosporins (TGC) and colistin, respectively, and are currently considered a major concern both in human and veterinary medicine. Little data on these resistance determinants prevalence in companion animal infections is available. The aim of this study was to determine the resistance profile of Escherichia coli isolated from pet infections, in Argentina, and to characterize the resistance mechanisms to TGC, as well as the presence of the plasmid-borne colistin resistance gene, mcr-1. A total of 54 E. coli isolates were collected from clinical samples in dogs and cats; from them, 20/54 (37%, CI 95: [24%; 51%]) displayed resistance to TGC. In this regard, thirteen pAmpC-producing isolates were positive for bla CMY-2 genes, whereas seven ESBL- producers harboured bla CTX-M-2 (n = 4), bla CTX-M-15 (n = 2) and bla CTX-M-14 (n = 1) genes. One E. coli strain (V80), isolated from a canine urinary tract infection, showed resistance to colistin (MIC = 8 μg/ml) and whole-genome sequencing analysis revealed co-occurrence of mcr-1.1, bla CTX-M-2 , aadA1, ant(2′')-Ia, catA1 and sul1 genes; the former being carried by a 60,587-bp IncI2 plasmid, previously reported in human colistin-resistant E. coli. E. coli V80 belonged to ST770 and the highly virulent phylogenetic group B2. In general, most of these multidrug-resistant isolates belonged to the phylogenetic group F (11/20) and to a lesser extent B2 (5/20), B1 (2/20), D (1/20) and E (1/20). In summary, CMY- and CTX-M-type β-lactamases may constitute the main TGC resistance mechanism in E. coli isolated from pet infections in Argentina, whereas dissemination of colistin resistance mechanism MCR-1 in the human-animal interface has been mediated by IncI2 plasmids.
Palabras clave:
ANTIBIOTIC RESISTANCE
,
CMY-2
,
CTX-M
,
ESBL
,
INCI2
,
MCR-1
,
PAMPC
,
PETS
,
SOUTH AMERICA
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Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Rumi, María Valeria; Mas, Javier; Elena, Alan Xavier; Cerdeira, Louise; Muñoz, Maria E.; et al.; Co-occurrence of clinically relevant β-lactamases and MCR-1 encoding genes in Escherichia coli from companion animals in Argentina; Elsevier Science; Veterinary Microbiology; 230; 3-2019; 228-234
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