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dc.contributor.author
Nadin, Silvina Beatriz  
dc.contributor.author
Nadin, Silvina Beatriz  
dc.contributor.author
Vargas Roig, Laura Maria  
dc.contributor.author
Vargas Roig, Laura Maria  
dc.contributor.author
Ciocca, Daniel Ramon  
dc.contributor.author
Ciocca, Daniel Ramon  
dc.date.available
2021-03-19T17:01:24Z  
dc.date.issued
2001-12  
dc.date.issued
2001-12  
dc.identifier.citation
Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186  
dc.identifier.citation
Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186  
dc.identifier.issn
0022-1554  
dc.identifier.issn
0022-1554  
dc.identifier.uri
http://hdl.handle.net/11336/128665  
dc.description.abstract
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.  
dc.description.abstract
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.  
dc.format
application/pdf  
dc.format
application/pdf  
dc.language.iso
eng  
dc.language.iso
eng  
dc.publisher
SAGE Publications  
dc.publisher
SAGE Publications  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
COMET ASSAY  
dc.subject
COMET ASSAY  
dc.subject
DNA DAMAGE  
dc.subject
DNA DAMAGE  
dc.subject
SILVER STAINING  
dc.subject
SILVER STAINING  
dc.subject.classification
Oncología  
dc.subject.classification
Oncología  
dc.subject.classification
Medicina Clínica  
dc.subject.classification
Medicina Clínica  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
A silver staining method for single-cell gel assay  
dc.title
A silver staining method for single-cell gel assay  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-01-18T15:47:16Z  
dc.journal.volume
49  
dc.journal.volume
49  
dc.journal.number
9  
dc.journal.number
9  
dc.journal.pagination
1183-1186  
dc.journal.pagination
1183-1186  
dc.journal.pais
Estados Unidos  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
California  
dc.journal.ciudad
California  
dc.description.fil
Fil: Nadin, Silvina Beatriz. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina  
dc.description.fil
Fil: Nadin, Silvina Beatriz. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina  
dc.description.fil
Fil: Vargas Roig, Laura Maria. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina  
dc.description.fil
Fil: Vargas Roig, Laura Maria. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina  
dc.description.fil
Fil: Ciocca, Daniel Ramon. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina  
dc.description.fil
Fil: Ciocca, Daniel Ramon. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina  
dc.journal.title
The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society  
dc.journal.title
The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/002215540104900912  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/002215540104900912  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1177/002215540104900912  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1177/002215540104900912  

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