Artículo
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains. The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.
A silver staining method for single-cell gel assay
Título:
A silver staining method for single-cell gel assay
Nadin, Silvina Beatriz
; Nadin, Silvina Beatriz
; Vargas Roig, Laura Maria
; Vargas Roig, Laura Maria
; Ciocca, Daniel Ramon
; Ciocca, Daniel Ramon
Fecha de publicación:
12/2001
12/2001
12/2001
Editorial:
SAGE Publications
SAGE Publications
SAGE Publications
Revista:
The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
ISSN:
0022-1554
0022-1554
0022-1554
Idioma:
Inglés
Inglés
Inglés
Tipo de recurso:
Artículo publicado
Resumen
Palabras clave:
COMET ASSAY
,
COMET ASSAY
,
DNA DAMAGE
,
DNA DAMAGE
,
SILVER STAINING
,
SILVER STAINING
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IMBECU)
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186
Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186
Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186
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