Artículo
Structural Characterization of Heparin-induced GAPDH Protofibrils Preventing α-synuclein Oligomeric Species Toxicity
Avila, Cesar Luis
; Torres Bugeau, Clarisa Maria
; Barbosa, Leandro R. S.; Morandé Sales, Elisa; Ouidja, Mohand O.; Socias, Sergio Benjamin
; Celej, Maria Soledad
; Raisman Vozari, Rita; Papy Garcia, Dulce; Itri, Rosangela; Chehin, Rosana Nieves
Fecha de publicación:
05/2014
Editorial:
American Society for Biochemistry and Molecular Biology
Revista:
Journal of Biological Chemistry
ISSN:
0021-9258
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme that has been associated with neurodegenerative diseases. GAPDH colocalizes with α-synuclein in amyloid aggregates in post-mortem tissue of patients with sporadic Parkinson disease and promotes the formation of Lewy body-like inclusions in cell culture. In a previous work, we showed that glycosaminoglycan-induced GAPDH prefibrillar species accelerate the conversion of α-synuclein to fibrils. However, it remains to be determined whether the interplay among glycosaminoglycans, GAPDH, and α-synuclein has a role in pathological states. Here, we demonstrate that the toxic effect exerted by α-synuclein oligomers in dopaminergic cell culture is abolished in the presence of GAPDH prefibrillar species. Structural analysis of prefibrillar GAPDH performed by small angle x-ray scattering showed a particle compatible with a protofibril. This protofibril is shaped as a cylinder 22 nm long and a cross-section diameter of 12 nm. Using biocomputational techniques, we obtained the first all-atom model of the GAPDH protofibril, which was validated by cross-linking coupled to mass spectrometry experiments. Because GAPDH can be secreted outside the cell where glycosaminoglycans are present, it seems plausible that GAPDH protofibrils could be assembled in the extracellular space kidnapping α-synuclein toxic oligomers. Thus, the role of GAPDH protofibrils in neuronal proteostasis must be considered. The data reported here could open alternative ways in the development of therapeutic strategies against synucleinopathies like Parkinson disease.
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Articulos(CCT - NOA SUR)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos(INQUINOA)
Articulos de INST.DE QUIMICA DEL NOROESTE
Articulos de INST.DE QUIMICA DEL NOROESTE
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Avila, Cesar Luis; Torres Bugeau, Clarisa Maria; Barbosa, Leandro R. S.; Morandé Sales, Elisa; Ouidja, Mohand O.; et al.; Structural Characterization of Heparin-induced GAPDH Protofibrils Preventing α-synuclein Oligomeric Species Toxicity; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 289; 20; 5-2014; 13838-13850
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