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dc.contributor.author
Lauthier, Juan José
dc.contributor.author
Ruybal, Paula
dc.contributor.author
Barroso, Paola Andrea
dc.contributor.author
Hashiguchi, Yoshihisa
dc.contributor.author
Marco, Jorge Diego
dc.contributor.author
Korenaga, Masataka
dc.date.available
2021-03-12T20:56:12Z
dc.date.issued
2020-01
dc.identifier.citation
Lauthier, Juan José; Ruybal, Paula; Barroso, Paola Andrea; Hashiguchi, Yoshihisa; Marco, Jorge Diego; et al.; Development of a Multilocus sequence typing (MLST) scheme for Pan-Leishmania; Elsevier Science; Acta Tropica; 201; 105189; 1-2020; 1-13
dc.identifier.issn
0001-706X
dc.identifier.uri
http://hdl.handle.net/11336/128295
dc.description.abstract
Since the description of the Leishmania genus, its identification and organization have been a challenge. A high number of molecular markers have been developed to resolve phylogenetic differences at the species level and for addressing key epidemiological and population genetics questions. Based on Multilocus enzyme electrophoresis (MLEE), Multilocus sequence typing (MLST) schemes have been developed using different gene candidates. From 38 original gene targets proposed by other authors, 27 of them were chosen. In silico selection was made by analyzing free access genomic sequence data of 33 Leishmania species, one Paraleishmania representative, and one outgroup, in order to select the best 15 loci. De novo amplifications and primers redesign of these 15 genes were analyzed over a panel of 20 reference strains and isolates. Phylogenetic analysis was made at every step. Two MLST schemes were selected. The first one was based on the analysis of three-gene fragments, and it is suitable for species assignment as well as basic phylogenetic studies. By the addition of seven-genes, an approach based on the analysis of ten-gene fragments was also proposed. This is the first work that two optimized MLST schemes have been suggested, validated against a phylogenetically diverse panel of Leishmania isolates. MLST is potentially a powerful phylogenetic approach, and most probably the new gold standard for Leishmania spp. characterization.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
MULTILOCUS SEQUENCE TYPING
dc.subject
OPTIMIZATION
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PAN-LEISHMANIA
dc.subject.classification
Parasitología
dc.subject.classification
Ciencias de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Development of a Multilocus sequence typing (MLST) scheme for Pan-Leishmania
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-01-27T19:55:17Z
dc.journal.volume
201
dc.journal.number
105189
dc.journal.pagination
1-13
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Lauthier, Juan José. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
dc.description.fil
Fil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón. Universidad Católica de Santiago de Guayaquil; Ecuador
dc.description.fil
Fil: Marco, Jorge Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
dc.description.fil
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón
dc.journal.title
Acta Tropica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0001706X19308447?via%3Dihub
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.actatropica.2019.105189
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