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Artículo

Development of a Multilocus sequence typing (MLST) scheme for Pan-Leishmania

Lauthier, Juan JoséIcon ; Ruybal, PaulaIcon ; Barroso, Paola AndreaIcon ; Hashiguchi, Yoshihisa; Marco, Jorge DiegoIcon ; Korenaga, Masataka
Fecha de publicación: 01/2020
Editorial: Elsevier Science
Revista: Acta Tropica
ISSN: 0001-706X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Parasitología

Resumen

Since the description of the Leishmania genus, its identification and organization have been a challenge. A high number of molecular markers have been developed to resolve phylogenetic differences at the species level and for addressing key epidemiological and population genetics questions. Based on Multilocus enzyme electrophoresis (MLEE), Multilocus sequence typing (MLST) schemes have been developed using different gene candidates. From 38 original gene targets proposed by other authors, 27 of them were chosen. In silico selection was made by analyzing free access genomic sequence data of 33 Leishmania species, one Paraleishmania representative, and one outgroup, in order to select the best 15 loci. De novo amplifications and primers redesign of these 15 genes were analyzed over a panel of 20 reference strains and isolates. Phylogenetic analysis was made at every step. Two MLST schemes were selected. The first one was based on the analysis of three-gene fragments, and it is suitable for species assignment as well as basic phylogenetic studies. By the addition of seven-genes, an approach based on the analysis of ten-gene fragments was also proposed. This is the first work that two optimized MLST schemes have been suggested, validated against a phylogenetically diverse panel of Leishmania isolates. MLST is potentially a powerful phylogenetic approach, and most probably the new gold standard for Leishmania spp. characterization.
Palabras clave: MULTILOCUS SEQUENCE TYPING , OPTIMIZATION , PAN-LEISHMANIA
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/128295
URL: https://www.sciencedirect.com/science/article/abs/pii/S0001706X19308447?via%3Dih
DOI: https://doi.org/10.1016/j.actatropica.2019.105189
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos(IPE)
Articulos de INST.DE PATOLOGIA EXPERIMENTAL
Citación
Lauthier, Juan José; Ruybal, Paula; Barroso, Paola Andrea; Hashiguchi, Yoshihisa; Marco, Jorge Diego; et al.; Development of a Multilocus sequence typing (MLST) scheme for Pan-Leishmania; Elsevier Science; Acta Tropica; 201; 105189; 1-2020; 1-13
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