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dc.contributor.author Lombardi, Maria Gabriela
dc.contributor.author Negroni, María Pía
dc.contributor.author Pelegrina, Laura Tatiana
dc.contributor.author Castro, Maria Ester
dc.contributor.author Fiszman, Gabriel Leon
dc.contributor.author Azar, María Eugenia
dc.contributor.author Cresta Morgado Carlos
dc.contributor.author Sales Maria Elena
dc.date.available 2015-07-16T18:47:08Z
dc.date.issued 2013-02
dc.identifier.citation Lombardi, Maria Gabriela; Negroni, María Pía; Pelegrina, Laura Tatiana; Castro, Maria Ester; Fiszman, Gabriel Leon; et al.; Autoantibodies against muscarinic receptors in breast cancer. Its role in tumor angiogenesis.; Public Library Science; Plos One; 8; 2; 2-2013; 57572-57579
dc.identifier.issn 1932-6203
dc.identifier.issn 10.1371/journal.pone.0057572
dc.identifier.uri http://hdl.handle.net/11336/1254
dc.description.abstract The presence of autoantibodies in cancer has become relevant in recent years. We demonstrated that autoantibodies purified from the sera of breast cancer patients activate muscarinic acetylcholine receptors in tumor cells. Immunoglobulin G (IgG) from breast cancer patients in T1N0Mx stage (tumor size¡Ü2 cm, without lymph node metastasis) mimics the action of the muscarinic agonist carbachol stimulating MCF-7 cell proliferation, migration and invasion. Angiogenesis is a central step in tumor progression because it promotes tumor invasion and metastatic spread. Vascular endothelial growth factor-A (VEGF-A) is the main angiogenic mediator, and its levels have been correlated with poor prognosis in cancer. The aim of the present work was to investigate the effect of T1N0Mx-IgG on the expression of VEGF-A, and the <em>in vivo</em> neovascular response triggered by MCF-7 cells, via muscarinic receptor activation. We demonstrated that T1N0Mx-IgG (10<sup><font size="2">−8</font></sup> M) and carbachol (10<sup><font size="2">−9</font></sup> M) increased the constitutive expression of VEGF-A in tumor cells, effect that was reverted by the muscarinic antagonist atropine. We also observed that T1N0Mx-IgG and carbachol enhanced the neovascular response produced by MCF-7 cells in the skin of NUDE mice. The action of IgG or carbachol was reduced in the presence of atropine. In conclusion, T1N0Mx-IgG and carbachol may promote VEGF-A production and neovascularization induced by breast tumor cells <em>via</em> muscarinic receptors activation. These effects may be accelerating breast tumor progression.
dc.format application/pdf
dc.language.iso eng
dc.publisher Public Library Science
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Autoantibodies
dc.subject muscarinic receptors
dc.subject angiogenesis
dc.subject breast cancer
dc.subject.classification Otras Medicina Básica
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Autoantibodies against muscarinic receptors in breast cancer. Its role in tumor angiogenesis.
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-03-30 10:35:44.97925-03
dc.journal.volume 8
dc.journal.number 2
dc.journal.pagination 57572-57579
dc.journal.pais Estados Unidos
dc.journal.ciudad San Francisco
dc.description.fil Fil: Lombardi, Maria Gabriela. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;
dc.description.fil Fil: Negroni, María Pía.
dc.description.fil Fil: Pelegrina, Laura Tatiana. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;
dc.description.fil Fil: Castro, Maria Ester. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;
dc.description.fil Fil: Fiszman, Gabriel Leon. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr.Ángel Roffo"; Argentina;
dc.description.fil Fil: Azar, María Eugenia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr.Ángel Roffo"; Argentina;
dc.description.fil Fil: Cresta Morgado Carlos. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr.Ángel Roffo"; Argentina;
dc.description.fil Fil: Sales Maria Elena. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;
dc.journal.title Plos One
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/23460876


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)