Artículo
Inhibitory effects of compounds isolated from Lepechinia meyenii on tyrosinase
Crespo, María Inés
; Funes Chabán, Macarena
; Lanza, Priscila Ailín; Joray, Mariana Belén
; Palacios, Sara Maria
; Vera, Domingo Mariano Adolfo
; Carpinella, María Cecilia
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Fecha de publicación:
03/2019
Editorial:
Pergamon-Elsevier Science Ltd
Revista:
Food and Chemical Toxicology
ISSN:
0278-6915
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
To contribute enzymatic browning inhibitors to the food industry and also extend knowledge about the phytochemical profile of the anti-tyrosinase plant Lepechinia meyenii, its ethanol extract was subjected to bioguided fractionation. Three hydroxycinnamic acids, p-coumaric acid (1), caffeic acid (2) and rosmarinic acid (3), were isolated as mainly responsible for its activity. Compounds 1, 2 and 3 showed themselves highly effective for inhibiting tyrosinase with IC 50 values of 0.30, 1.50 and 4.14 μM, respectively, for monophenolase activity and 0.62, 2.30 and 8.59 μM, respectively for diphenolase activity. This is the first report describing the isolation of the compounds causing the tyrosinase inhibitory activity of L. meyenii extract. The inhibitory kinetics of 1–3 using both L-tyrosine and L-DOPA as substrates was investigated and the results obtained were discussed at molecular level by docking analysis. The resulting compounds 1–3 and a phenolic-enriched fraction of the extract, 2.9-fold more active than the starting material, may be suitable as non-toxic and inexpensive alternatives for the control of deleterious enzymatic darkening.
Palabras clave:
LEPECHINIA MEYENII
,
MOLECULAR DOCKING
,
PHENOLIC ACIDS
,
TYROSINASE INHIBITORS
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Licencia
Identificadores
Colecciones
Articulos(INBIOTEC)
Articulos de INSTITUTO DE INV. EN BIODIVERSIDAD Y BIOTECNOLOGIA
Articulos de INSTITUTO DE INV. EN BIODIVERSIDAD Y BIOTECNOLOGIA
Articulos(IRNASUS)
Articulos de INSTITUTO DE INVESTIGACIONES EN RECURSOS NATURALES Y SUSTENTABILIDAD JOSE SANCHEZ LABRADOR S.J.
Articulos de INSTITUTO DE INVESTIGACIONES EN RECURSOS NATURALES Y SUSTENTABILIDAD JOSE SANCHEZ LABRADOR S.J.
Citación
Crespo, María Inés; Funes Chabán, Macarena; Lanza, Priscila Ailín; Joray, Mariana Belén; Palacios, Sara Maria; et al.; Inhibitory effects of compounds isolated from Lepechinia meyenii on tyrosinase; Pergamon-Elsevier Science Ltd; Food and Chemical Toxicology; 125; 3-2019; 383-391
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