Artículo
Exploring lectin-like activity of the S-layer protein of Lactobacillus acidophilus ATCC 4356
Fina Martin, Joaquina
; Palomino, Maria Mercedes
; Cutine, Anabela María
; Modenutti, Carlos Pablo
; Fernández Do Porto, Darío Augusto
; Allievi, Mariana Caludia
; Zanini, Sofía Helena; Mariño, Karina Valeria
; Barquero, Andrea Alejandra
; Ruzal, Sandra Mónica
Fecha de publicación:
06/2019
Editorial:
Springer
Revista:
Applied Microbiology and Biotechnology
ISSN:
0175-7598
e-ISSN:
1432-0614
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The surface layer (S-layer) protein of Lactobacillus acidophilus is a crystalline array of self-assembling, proteinaceous subunits non-covalently bound to the outmost bacterial cell wall envelope and is involved in the adherence of bacteria to host cells. We have previously described that the S-layer protein of L. acidophilus possesses anti-viral and anti-bacterial properties. In this work, we extracted and purified S-layer proteins from L. acidophilus ATCC 4356 cells to study their interaction with cell wall components from prokaryotic (i.e., peptidoglycan and lipoteichoic acids) and eukaryotic origin (i.e., mucin and chitin), as well as with viruses, bacteria, yeast, and blood cells. Using chimeric S-layer fused to green fluorescent protein (GFP) from different parts of the protein, we analyzed their binding capacity. Our results show that the C-terminal part of the S-layer protein presents lectin-like activity, interacting with different glycoepitopes. We further demonstrate that lipoteichoic acid (LTA) serves as an anchor for the S-layer protein. Finally, a structure for the C-terminal part of S-layer and possible binding sites were predicted by a homology-based model.
Palabras clave:
LACTOBACILLUS
,
LECTIN ACTIVITY
,
S-LAYER
,
Lactobacillus acidophilus
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Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Fina Martin, Joaquina; Palomino, Maria Mercedes; Cutine, Anabela María; Modenutti, Carlos Pablo; Fernández Do Porto, Darío Augusto; et al.; Exploring lectin-like activity of the S-layer protein of Lactobacillus acidophilus ATCC 4356; Springer; Applied Microbiology and Biotechnology; 103; 12; 6-2019; 4839-4857
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