Artículo
Changes in cardiac Aquaporin expression during aortic valve replacement surgery with cardiopulmonary bypass
Politi, María Teresa; Ochoa, Federico Claudio; Netti, Vanina Alejandra
; Ferreyra, Raúl; Bortman, Guillermo; Sanjuan, Norberto Aníbal
; Morales, Celina; Piazza, Antonio; Capurro, Claudia Graciela
Fecha de publicación:
09/2019
Editorial:
Elsevier Science
Revista:
European Journal Of Cardio-thoracic Surgery
ISSN:
1010-7940
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
OBJECTIVES: Cardiopulmonary bypass (CPB) use is an essential strategy for many cardiovascular surgeries. However, its use and duration have been associated with a higher rate of postoperative complications, such as low cardiac output syndrome due to myocardial oedema and dysfunction. Though Aquaporin water channels have been implicated in myocardial water balance, their specific role in this clinical scenario has not been established. METHODS: In a consecutive study of 17 patients with severe aortic stenosis undergoing aortic valve replacement surgery, 2 myocardial biopsies of the left ventricle were taken: 1 before and 1 after CPB use. Sociodemographic, clinical and laboratory data were collected. Western blot and immunohistochemistry studies were performed. RESULTS: After CPB use, there was a mean increase of ∼62% in Aquaporin 1 protein levels (P = 0.001) and a mean reduction of ∼38% in Aquaporin 4 protein levels (P = 0.030). In immunohistochemistry assays, Aquaporin 1 was found lining small blood vessels, while Aquaporin 4 formed a circular label in cardiomyocytes. There were no changes in the localization of either protein following CPB use. During the observed on-pump time interval, there was a 1.7%/min mean increase in Aquaporin 1 (P = 0.021) and a 2.5%/min mean decrease in Aquaporin 4 (P = 0.018). Myocardial interstitial oedema increased by 42% (95% confidence interval 31-54%) after CPB use. Patients who developed low cardiac output syndrome were in the upper half of the median percentage change of Aquaporin expression. CONCLUSION: Time-dependent changes in cardiac Aquaporin expression may be associated with myocardial oedema and dysfunction related to CPB use.
Palabras clave:
AORTIC VALVE
,
AQUAPORINS
,
CARDIOPULMONARY BYPASS
,
HUMANS
,
MYOCARDIUM
,
OEDEMA
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Licencia
Identificadores
Colecciones
Articulos(IFIBIO HOUSSAY)
Articulos de INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Articulos de INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Politi, María Teresa; Ochoa, Federico Claudio; Netti, Vanina Alejandra; Ferreyra, Raúl; Bortman, Guillermo; et al.; Changes in cardiac Aquaporin expression during aortic valve replacement surgery with cardiopulmonary bypass; Elsevier Science; European Journal Of Cardio-thoracic Surgery; 57; 3; 9-2019; 556-564
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