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dc.contributor.author
Tito, Florencia Rocío  
dc.contributor.author
Pepe, Alfonso  
dc.contributor.author
Tonón, Claudia Virginia  
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Daleo, Gustavo Raúl  
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Guevara, María Gabriela  
dc.date.available
2020-10-09T20:08:37Z  
dc.date.issued
2020-01-23  
dc.identifier.citation
Tito, Florencia Rocío; Pepe, Alfonso; Tonón, Claudia Virginia; Daleo, Gustavo Raúl; Guevara, María Gabriela; Determination and characterisation of milk-clotting activity of two Solanum tuberosum aspartic proteases (StAPs); Elsevier; International Dairy Journal; 104; 23-1-2020; 104645  
dc.identifier.issn
0958-6946  
dc.identifier.uri
http://hdl.handle.net/11336/115700  
dc.description.abstract
To identify new coagulant enzymes as rennet substitutes, the aim of this study was to determine and to characterise the milk-clotting activity (MCA) of two potato aspartic proteases (StAPs). Both enzymes exhibited MCA in a dose-dependent manner. Optimum MCA values were determined at pH 5 and 30 °C. The ability of StAPs to degrade casein subunits was also evaluated. β-Casein was preferentially hydrolysed by both StAPs, followed by αS-casein and, to a lesser extent, κ-casein. These results confirmed the suitability of StAPs for producing curd and the possibility of using these proteases in artisanal cheese production.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Milk clotting  
dc.subject
Plant proteases  
dc.subject
Solanum tuberosum  
dc.subject.classification
Alimentos y Bebidas  
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Otras Ingenierías y Tecnologías  
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INGENIERÍAS Y TECNOLOGÍAS  
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Físico-Química, Ciencia de los Polímeros, Electroquímica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Determination and characterisation of milk-clotting activity of two Solanum tuberosum aspartic proteases (StAPs)  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-07-01T16:10:06Z  
dc.journal.volume
104  
dc.journal.pagination
104645  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Tito, Florencia Rocío. Universidad Nacional de Mar del Plata; Argentina  
dc.description.fil
Fil: Pepe, Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina  
dc.description.fil
Fil: Tonón, Claudia Virginia. Universidad Nacional de Mar del Plata; Argentina  
dc.description.fil
Fil: Daleo, Gustavo Raúl. Universidad Nacional de Mar del Plata; Argentina  
dc.description.fil
Fil: Guevara, María Gabriela. Universidad Nacional de Mar del Plata; Argentina  
dc.journal.title
International Dairy Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.idairyj.2020.104645  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0958694620300157?via%3Dihub