Artículo
Determination and characterisation of milk-clotting activity of two Solanum tuberosum aspartic proteases (StAPs)
Tito, Florencia Rocío; Pepe, Alfonso
; Tonón, Claudia Virginia; Daleo, Gustavo Raúl; Guevara, María Gabriela
Fecha de publicación:
23/01/2020
Editorial:
Elsevier
Revista:
International Dairy Journal
ISSN:
0958-6946
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
To identify new coagulant enzymes as rennet substitutes, the aim of this study was to determine and to characterise the milk-clotting activity (MCA) of two potato aspartic proteases (StAPs). Both enzymes exhibited MCA in a dose-dependent manner. Optimum MCA values were determined at pH 5 and 30 °C. The ability of StAPs to degrade casein subunits was also evaluated. β-Casein was preferentially hydrolysed by both StAPs, followed by αS-casein and, to a lesser extent, κ-casein. These results confirmed the suitability of StAPs for producing curd and the possibility of using these proteases in artisanal cheese production.
Palabras clave:
Milk clotting
,
Plant proteases
,
Solanum tuberosum
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Articulos(INTEMA)
Articulos de INST.DE INV.EN CIENCIA Y TECNOL.MATERIALES (I)
Articulos de INST.DE INV.EN CIENCIA Y TECNOL.MATERIALES (I)
Citación
Tito, Florencia Rocío; Pepe, Alfonso; Tonón, Claudia Virginia; Daleo, Gustavo Raúl; Guevara, María Gabriela; Determination and characterisation of milk-clotting activity of two Solanum tuberosum aspartic proteases (StAPs); Elsevier; International Dairy Journal; 104; 23-1-2020; 104645
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