Artículo
Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation
Medina, Vanina Araceli
; Massari, Noelia Andrea
; Cricco, Graciela Patricia; Martín, Gabriela A.; Bergoc, Rosa Maria
; Rivera, Elena Susana
Fecha de publicación:
06/2009
Editorial:
Elsevier Science Inc
Revista:
Free Radical Biology and Medicine
ISSN:
0891-5849
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Histamine is a recognized growth factor in melanoma, and exogenous histamine produces a dual effect on proliferation. We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. To investigate the mechanism by which histamine inhibits proliferation of WM35 human melanoma cells, we have studied the involvement of histamine in reactive oxygen species production and antioxidant enzyme regulation in these cells. Results indicate that histamine treatment (10 μM) significantly increased hydrogen peroxide levels, whereas it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction in catalase activity. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation, and various treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrate that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells.
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Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Medina, Vanina Araceli; Massari, Noelia Andrea; Cricco, Graciela Patricia; Martín, Gabriela A.; Bergoc, Rosa Maria; et al.; Involvement of hydrogen peroxide in histamine-induced modulation of WM35 human malignant melanoma cell proliferation; Elsevier Science Inc; Free Radical Biology and Medicine; 46; 11; 6-2009; 1510-1515
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