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dc.contributor.author
Monteserin, Johana
dc.contributor.author
Paul, Roxana Elizabeth
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López, Beatriz Graciela
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Cnockaert, M.
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Tortoli, E
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Méndez, C.
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García, M. J.
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Palomino, Juan Carlos
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Vandamme, P.
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Ritacco, Gloria Viviana
dc.contributor.author
Martin, A.
dc.date.available
2020-09-14T17:01:04Z
dc.date.issued
2016-09
dc.identifier.citation
Monteserin, Johana; Paul, Roxana Elizabeth; López, Beatriz Graciela; Cnockaert, M.; Tortoli, E; et al.; Combined approach to the identification of clinically infrequent non-tuberculous mycobacteria in Argentina; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 20; 9; 9-2016; 1257-1262
dc.identifier.issn
1027-3719
dc.identifier.uri
http://hdl.handle.net/11336/113936
dc.description.abstract
SETTING: Over 150 potentially pathogenic non-tuberculous mycobacteria (NTM) species have been described, posing an onerous challenge for clinical laboratory diagnosis. OBJECTIVE: To evaluate different approaches for the identification of 40 clinically relevant NTM isolates whose species were not reliably identified using our routine diagnostic workflow comprising phenotypic tests and hsp65 polymerase chain reaction restriction analysis. DESIGN: We used 1) sequencing analysis of four conserved gene targets: 16S rRNA, rpoB, hsp65 and sodA; 2) two commercial reverse hybridisation assays; and 3) protein analysis using matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS). RESULTS: Combined, but not individual, sequence analysis allowed reliable species identification for 30/ 40 (75%) isolates, including species previously unknown to be circulating in Argentina. Commercial kits outperformed our routine identification in only 5/35 isolates, and misclassified many more. MALDI-TOF MS accurately identified species in 22/36 (61%) isolates and did not misidentify any. CONCLUSIONS: Commercial kits did not resolve the problem of species of NTM isolates that elude identification. Combined DNA sequence analysis was the approach of choice. MALDI-TOF MS shows promise as a powerful, rapid and accessible tool for the rapid identification of clinically relevant NTM in the diagnostic laboratory, and its accuracy can be maximised by building up a customised NTM spectrum database.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
International Union Against Tuberculosis and Lung Disease
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DNA PROBES
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DNA SEQUENCE ANALYSIS
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MATRIX-ASSISTED LASER DESORPTION-IONIZATION MASS SPECTROMETRY (MALDI-TOF MS)
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MYCOBACTERIOSIS
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NUCLEIC ACID HYBRIDISATION
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Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Combined approach to the identification of clinically infrequent non-tuberculous mycobacteria in Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-11T18:51:33Z
dc.journal.volume
20
dc.journal.number
9
dc.journal.pagination
1257-1262
dc.journal.pais
Francia
dc.journal.ciudad
Paris
dc.description.fil
Fil: Monteserin, Johana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Paul, Roxana Elizabeth. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
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Fil: López, Beatriz Graciela. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cnockaert, M.. University of Ghent; Bélgica
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Fil: Tortoli, E. Istituto di Ricovero; Italia. Cura a Carattere Scientifico San Raffaele Scientific Institute; Italia
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Fil: Méndez, C.. Universidad Autónoma de Madrid; España
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Fil: García, M. J.. Universidad Autónoma de Madrid; España
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Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
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Fil: Vandamme, P.. University of Ghent; Bélgica
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Fil: Ritacco, Gloria Viviana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Martin, A.. University of Ghent; Bélgica
dc.journal.title
International Journal of Tuberculosis and Lung Disease
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.5588/ijtld.16.0122
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/iuatld/ijtld/2016/00000020/00000009/art00024;jsessionid=u7gb5p1q7mp2.x-ic-live-03
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