Artículo
Combined approach to the identification of clinically infrequent non-tuberculous mycobacteria in Argentina
Monteserin, Johana
; Paul, Roxana Elizabeth; López, Beatriz Graciela; Cnockaert, M.; Tortoli, E; Méndez, C.; García, M. J.; Palomino, Juan Carlos; Vandamme, P.; Ritacco, Gloria Viviana
; Martin, A.
Fecha de publicación:
09/2016
Editorial:
International Union Against Tuberculosis and Lung Disease
Revista:
International Journal of Tuberculosis and Lung Disease
ISSN:
1027-3719
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
SETTING: Over 150 potentially pathogenic non-tuberculous mycobacteria (NTM) species have been described, posing an onerous challenge for clinical laboratory diagnosis. OBJECTIVE: To evaluate different approaches for the identification of 40 clinically relevant NTM isolates whose species were not reliably identified using our routine diagnostic workflow comprising phenotypic tests and hsp65 polymerase chain reaction restriction analysis. DESIGN: We used 1) sequencing analysis of four conserved gene targets: 16S rRNA, rpoB, hsp65 and sodA; 2) two commercial reverse hybridisation assays; and 3) protein analysis using matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS). RESULTS: Combined, but not individual, sequence analysis allowed reliable species identification for 30/ 40 (75%) isolates, including species previously unknown to be circulating in Argentina. Commercial kits outperformed our routine identification in only 5/35 isolates, and misclassified many more. MALDI-TOF MS accurately identified species in 22/36 (61%) isolates and did not misidentify any. CONCLUSIONS: Commercial kits did not resolve the problem of species of NTM isolates that elude identification. Combined DNA sequence analysis was the approach of choice. MALDI-TOF MS shows promise as a powerful, rapid and accessible tool for the rapid identification of clinically relevant NTM in the diagnostic laboratory, and its accuracy can be maximised by building up a customised NTM spectrum database.
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Monteserin, Johana; Paul, Roxana Elizabeth; López, Beatriz Graciela; Cnockaert, M.; Tortoli, E; et al.; Combined approach to the identification of clinically infrequent non-tuberculous mycobacteria in Argentina; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 20; 9; 9-2016; 1257-1262
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