Mostrar el registro sencillo del ítem
dc.contributor.author
Ben Achour, Nahed
dc.contributor.author
Power, Pablo
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Mercuri, Pablo Alberto
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Moussa, Mohamed Ben
dc.contributor.author
Moreno, Galleni
dc.contributor.author
Omrane. Belhadj
dc.date.available
2020-06-23T15:39:33Z
dc.date.issued
2012-03
dc.identifier.citation
Ben Achour, Nahed; Power, Pablo; Mercuri, Pablo Alberto; Moussa, Mohamed Ben; Moreno, Galleni; et al.; First detection of a transferable bla CTX-M-14b gene in a Klebsiella pneumoniae clinical isolate from Tunisia and analysis of its genetic context; Inst Microbiologia; Annals Of Microbiology; 62; 4; 3-2012; 1737-1742
dc.identifier.issn
1590-4261
dc.identifier.uri
http://hdl.handle.net/11336/107928
dc.description.abstract
Klebsiella pneumoniae ML2508 was isolated from a patient at the surgery unit of the Military Hospital (Hôpital Militaire de Tunis), Tunisia. It was identified as a producer of extended-spectrum β-lactamases (ESBLs) by the double-disk synergy test. The β-lactamases produced by the strain were characterized by isoelectric focusing, determination of the specific activities against penicillins and cephalosporins, determination of the inhibitory concentration required to inhibit 50% of enzyme activity (IC50), and the inhibition effect of EDTA on putative metallo-β- lactamases. The crude extract of K. pneumoniae ML2508 contains five different β-lactamases with pI 5.5, 7.3, 7.6, 8.1, and 8.6. Only the β-lactamase with pI 8.1 was transferred by transformation and conjugation experiments. Molecular characterization of these genes was performed by PCR and sequencing. The four chromosomal β-lactamases are TEM (pI 5.5), 2 SHV (pI 7.3 and 7.6), and CTX-M-28 (pI 8.6). The β-lactamase with pI 8.1 was encoded by blaCTX-M-14b gene located on a 50-kb highly conjugative plasmid. The study of the genetic context of blaCTX-M-14b was realized by PCR-mapping and DNA sequencing. A novel variant of tnpISEcp1 designated ISEcp1C was detected upstream of the gene.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Inst Microbiologia
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
b-lactamase
dc.subject
PCR-mapping
dc.subject
DNA sequencing
dc.subject.classification
Otras Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
First detection of a transferable bla CTX-M-14b gene in a Klebsiella pneumoniae clinical isolate from Tunisia and analysis of its genetic context
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-11T18:16:54Z
dc.journal.volume
62
dc.journal.number
4
dc.journal.pagination
1737-1742
dc.journal.pais
Italia
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Milan
dc.description.fil
Fil: Ben Achour, Nahed. Faculte Des Sciences de Tunis; Túnez
dc.description.fil
Fil: Power, Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Université de Liège; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.fil
Fil: Mercuri, Pablo Alberto. Université de Liège; Bélgica
dc.description.fil
Fil: Moussa, Mohamed Ben. Hôpital Militaire de Tunis, ; Túnez
dc.description.fil
Fil: Moreno, Galleni. Université de Liège; Bélgica
dc.description.fil
Fil: Omrane. Belhadj. Faculté des Sciences de Tunis; Túnez
dc.journal.title
Annals Of Microbiology
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s13213-012-0430-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s13213-012-0430-y
Archivos asociados