Artículo
First detection of a transferable bla CTX-M-14b gene in a Klebsiella pneumoniae clinical isolate from Tunisia and analysis of its genetic context
Ben Achour, Nahed; Power, Pablo
; Mercuri, Pablo Alberto; Moussa, Mohamed Ben; Moreno, Galleni; Omrane. Belhadj
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Fecha de publicación:
03/2012
Editorial:
Inst Microbiologia
Revista:
Annals Of Microbiology
ISSN:
1590-4261
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Klebsiella pneumoniae ML2508 was isolated from a patient at the surgery unit of the Military Hospital (Hôpital Militaire de Tunis), Tunisia. It was identified as a producer of extended-spectrum β-lactamases (ESBLs) by the double-disk synergy test. The β-lactamases produced by the strain were characterized by isoelectric focusing, determination of the specific activities against penicillins and cephalosporins, determination of the inhibitory concentration required to inhibit 50% of enzyme activity (IC50), and the inhibition effect of EDTA on putative metallo-β- lactamases. The crude extract of K. pneumoniae ML2508 contains five different β-lactamases with pI 5.5, 7.3, 7.6, 8.1, and 8.6. Only the β-lactamase with pI 8.1 was transferred by transformation and conjugation experiments. Molecular characterization of these genes was performed by PCR and sequencing. The four chromosomal β-lactamases are TEM (pI 5.5), 2 SHV (pI 7.3 and 7.6), and CTX-M-28 (pI 8.6). The β-lactamase with pI 8.1 was encoded by blaCTX-M-14b gene located on a 50-kb highly conjugative plasmid. The study of the genetic context of blaCTX-M-14b was realized by PCR-mapping and DNA sequencing. A novel variant of tnpISEcp1 designated ISEcp1C was detected upstream of the gene.
Palabras clave:
b-lactamase
,
PCR-mapping
,
DNA sequencing
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Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Ben Achour, Nahed; Power, Pablo; Mercuri, Pablo Alberto; Moussa, Mohamed Ben; Moreno, Galleni; et al.; First detection of a transferable bla CTX-M-14b gene in a Klebsiella pneumoniae clinical isolate from Tunisia and analysis of its genetic context; Inst Microbiologia; Annals Of Microbiology; 62; 4; 3-2012; 1737-1742
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