Ethanol and protein production from minimally processed biomass of a genetically-modified cyanobacterium over-accumulating sucrose

Abstract

One of the main bottlenecks of a microalgal or cyanobacterial biomass biorefinery is the separation of differentuseful fractions using simple, low energy-consuming, cost-effective, and scalable separation processes. Althoughthe carbohydrates-rich biomass of these microorganisms presents clear advantages over conventional terrestrialcrops as feedstocks for ethanol, it still requires acid and/or enzymatic hydrolysis for efficient fermentation. Here,we show the genetic modification of carbohydrates partitioning in afilamentous cyanobacterium towards theaccumulation of sucrose up to 10% (w/w) as a readily fermentable feedstock. We optimized two methods for thepreparation of concentrated sucrose syrups, which were efficiently converted into ethanol by yeasts, without theneed of additional pretreatments. Biomass drying and milling, followed by aqueous extraction of sugars andproteins, and the recovery of proteins by short pulses of heat, kept the value of sugars as a feedstock for ethanoland protein for feed supplements within a cost-effective biomass biorefinery.