Artículo
Ethanol and protein production from minimally processed biomass of a genetically-modified cyanobacterium over-accumulating sucrose
Fecha de publicación:
02/2019
Editorial:
Elsevier
Revista:
Bioresource Technology Reports
ISSN:
2589-014X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
One of the main bottlenecks of a microalgal or cyanobacterial biomass biorefinery is the separation of differentuseful fractions using simple, low energy-consuming, cost-effective, and scalable separation processes. Althoughthe carbohydrates-rich biomass of these microorganisms presents clear advantages over conventional terrestrialcrops as feedstocks for ethanol, it still requires acid and/or enzymatic hydrolysis for efficient fermentation. Here,we show the genetic modification of carbohydrates partitioning in afilamentous cyanobacterium towards theaccumulation of sucrose up to 10% (w/w) as a readily fermentable feedstock. We optimized two methods for thepreparation of concentrated sucrose syrups, which were efficiently converted into ethanol by yeasts, without theneed of additional pretreatments. Biomass drying and milling, followed by aqueous extraction of sugars andproteins, and the recovery of proteins by short pulses of heat, kept the value of sugars as a feedstock for ethanoland protein for feed supplements within a cost-effective biomass biorefinery.
Palabras clave:
ANABAENA SP.
,
BIOETHANOL
,
GENETIC ENGINEERING
,
SUCROSE
,
BIOFUELS
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Articulos(INBIOTEC)
Articulos de INSTITUTO DE INV. EN BIODIVERSIDAD Y BIOTECNOLOGIA
Articulos de INSTITUTO DE INV. EN BIODIVERSIDAD Y BIOTECNOLOGIA
Citación
Sanz Smachetti, María Eugenia; Perez Cenci, Macarena; Salerno, Graciela Lidia; Curatti, Leonardo; Ethanol and protein production from minimally processed biomass of a genetically-modified cyanobacterium over-accumulating sucrose; Elsevier; Bioresource Technology Reports; 5; 2-2019; 230-237
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