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dc.contributor.author
Hirohashi, Noritaka
dc.contributor.author
Gerton, George L.
dc.contributor.author
Buffone, Mariano Gabriel
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dc.date.available
2016-12-28T17:14:09Z
dc.date.issued
2011-07
dc.identifier.citation
Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel; Video imaging of the sperm acrosome reaction during in vitro fertilization; Landes Bioscience; Communicative and Integrative Biology; 4; 4; 7-2011; 471-476
dc.identifier.issn
1942-0889
dc.identifier.uri
http://hdl.handle.net/11336/10487
dc.description.abstract
Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Landes Bioscience
dc.rights
info:eu-repo/semantics/openAccess
dc.rights
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acrosomal Exocytosis
dc.subject
Sperm
dc.subject
Fertilization
dc.subject
Acrosome
dc.subject.classification
Biología Reproductiva
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Video imaging of the sperm acrosome reaction during in vitro fertilization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-12-27T20:13:05Z
dc.identifier.eissn
1942-0889
dc.journal.volume
4
dc.journal.number
4
dc.journal.pagination
471-476
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Austin
dc.description.fil
Fil: Hirohashi, Noritaka. Ochanomizu University; Japón
dc.description.fil
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos
dc.description.fil
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.journal.title
Communicative and Integrative Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4161/cib.4.4.15636
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181525/
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