Artículo
Video imaging of the sperm acrosome reaction during in vitro fertilization
Fecha de publicación:
07/2011
Editorial:
Landes Bioscience
Revista:
Communicative and Integrative Biology
ISSN:
1942-0889
e-ISSN:
1942-0889
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.
Palabras clave:
Acrosomal Exocytosis
,
Sperm
,
Fertilization
,
Acrosome
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Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel; Video imaging of the sperm acrosome reaction during in vitro fertilization; Landes Bioscience; Communicative and Integrative Biology; 4; 4; 7-2011; 471-476
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