Heterogeneity in the antibody response to foot-and-mouth disease primo vaccinated calves

Abstract

Foot-and-mouth disease (FMD) vaccines are routinely used as effective control tools in large regions worldwide and to limit outbreaks during epidemics. Vaccine-induced protection in cattle has been largely correlated to the FMD virus (FMDV)-specific antibodies. Genetic control of cattle immune adaptive responses has been demonstrated only for peptide antigens derived from FMDV structural proteins. Here, we quantify the heterogeneity in the antibody response of cattle primo-vaccinated against FMD and study its association with the genetic background in Holstein and Jersey sires. A total of 377 FMDV seronegative calves (122 and 255calves from 16 and 15 Holstein and Jersey sires, respectively) were included in the study. Samples were taken the day prior to primovaccination and 45 days post-vaccination (dpv). Animals received commercial tetravalent FMD single-emulsion oil vaccines formulated with inactivated FMDV. Total FMDVspecific antibody responses were studied against 3 viral strains included in the vaccine and antibody titers were determined by liquid phase blocking-ELISA. Three linear hierarchical mixed regression models, one for each strain, were formulated to assess the heterogeneity in the immune responses to vaccination. The dependent variables were the antibody titers induced against each FMDV strain at 45 dpv, whereas sire?s ?breed? was included as a fixed effect, ?sire? was included as a random effect and ?farm? was considered as a hierarchical factor to account for lack of independence of within herd measurements. A significant association was found between anti-FMDV antibody responses and sire?s breed, with lower immune responses found in the Jersey sires? offspring compared to those from Holstein sires. No significant intra-breed variation was detected. In addition, farm management practices were similar in this study and results of the serological assays were shown to be repeatable. It therefore seems plausible that differences in the immune response may be expected in the event of a mass vaccination campaigns.