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dc.contributor.author
Paz, Rosalia Cristina
dc.contributor.author
Rocco, Ruben Anibal
dc.contributor.author
Bermont Jiménez, Juan Francisco
dc.contributor.author
Rodríguez y Domínguez Kessler, Margarita
dc.contributor.author
Becerra Flora, Alicia
dc.contributor.author
Menendez, Ana Bernardina
dc.contributor.author
Ruiz, Oscar Adolfo
dc.date.available
2020-04-03T16:42:25Z
dc.date.issued
2014-06
dc.identifier.citation
Paz, Rosalia Cristina; Rocco, Ruben Anibal; Bermont Jiménez, Juan Francisco; Rodríguez y Domínguez Kessler, Margarita; Becerra Flora, Alicia; et al.; Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 82; 6-2014; 279-288
dc.identifier.issn
0981-9428
dc.identifier.uri
http://hdl.handle.net/11336/101850
dc.description.abstract
Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L. tenuis plants, subtractive cDNA libraries were generated from leaves and roots of these plants. Total RNAs of non-stressed plants (pH 5.8; E.C. 1.2), and plants stressed by the addition of 10 mM of NaHCO3 (pH 9.0; E.C. 1.9), were used as source of the driver and the tester samples, respectively. RNA samples were collected after 14 and 28 days of treatment. A total of 158 unigenes from leaves and 92 unigenes from roots were obtained and classified into 11 functional categories. Unigenes from these categories (4 for leaves and 8 for roots), that were related with nutrient metabolism and oxidative stress relief were selected, and their differential expression analyzed by qRT-PCR. These genes were found to be differentially expressed in a time dependent manner in L. tenuis during the alkaline stress application. Data generated from this study will contribute to the understanding of the general molecular mechanisms associated to plant tolerance under long-term alkaline stress in plants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier France-editions Scientifiques Medicales Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lotus tenuis
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Alkaline stress
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Adaptive response
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Long-term response
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Root and shoot tissues
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Suppressive Subtractive Hybridization
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Stress induced transcripts
dc.subject.classification
Otras Biotecnología Agropecuaria
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Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-02T14:59:26Z
dc.journal.volume
82
dc.journal.pagination
279-288
dc.journal.pais
Francia
dc.journal.ciudad
Paris
dc.description.fil
Fil: Paz, Rosalia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Centro de Investigaciones de la Geosfera y Biosfera. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones de la Geosfera y Biosfera; Argentina
dc.description.fil
Fil: Rocco, Ruben Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Bermont Jiménez, Juan Francisco. Instituto Potosino de Investigación Científica y Tecnológica; México
dc.description.fil
Fil: Rodríguez y Domínguez Kessler, Margarita. Universidad Autónoma de San Luis Potosí.; México
dc.description.fil
Fil: Becerra Flora, Alicia. Instituto Potosino de Investigación Científica y Tecnológica; México
dc.description.fil
Fil: Menendez, Ana Bernardina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
dc.description.fil
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.journal.title
Plant Physiology and Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plaphy.2014.06.009
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0981942814001909
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