Artículo
Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress
Paz, Rosalia Cristina
; Rocco, Ruben Anibal
; Bermont Jiménez, Juan Francisco; Rodríguez y Domínguez Kessler, Margarita; Becerra Flora, Alicia; Menendez, Ana Bernardina
; Ruiz, Oscar Adolfo
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Fecha de publicación:
06/2014
Editorial:
Elsevier France-editions Scientifiques Medicales Elsevier
Revista:
Plant Physiology and Biochemistry
ISSN:
0981-9428
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L. tenuis plants, subtractive cDNA libraries were generated from leaves and roots of these plants. Total RNAs of non-stressed plants (pH 5.8; E.C. 1.2), and plants stressed by the addition of 10 mM of NaHCO3 (pH 9.0; E.C. 1.9), were used as source of the driver and the tester samples, respectively. RNA samples were collected after 14 and 28 days of treatment. A total of 158 unigenes from leaves and 92 unigenes from roots were obtained and classified into 11 functional categories. Unigenes from these categories (4 for leaves and 8 for roots), that were related with nutrient metabolism and oxidative stress relief were selected, and their differential expression analyzed by qRT-PCR. These genes were found to be differentially expressed in a time dependent manner in L. tenuis during the alkaline stress application. Data generated from this study will contribute to the understanding of the general molecular mechanisms associated to plant tolerance under long-term alkaline stress in plants.
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Articulos(CCT - SAN JUAN)
Articulos de CENTRO CIENTIFICO TECNOLOGICO CONICET - SAN JUAN
Articulos de CENTRO CIENTIFICO TECNOLOGICO CONICET - SAN JUAN
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Paz, Rosalia Cristina; Rocco, Ruben Anibal; Bermont Jiménez, Juan Francisco; Rodríguez y Domínguez Kessler, Margarita; Becerra Flora, Alicia; et al.; Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 82; 6-2014; 279-288
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