Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir

Gerbino, Oscar EstebanIcon ; Carasi, PaulaIcon ; Araujo Andrade, Cuauhtémoc; Tymczyszyn, Emma ElizabethIcon ; Gomez Zavaglia, AndreaIcon
Fecha de publicación: 02/2015
Editorial: Springer
Revista: World Journal of Microbiology
ISSN: 0959-3993
e-ISSN: 1573-0972
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ingenierías y Tecnologías

Resumen

The role of S-layer proteins (SLP) on the Pb2+ sequestrant capacity by Lactobacillus kefir CIDCA 8348 and JCM 5818 was investigated. Cultures in the stationary phase were treated with proteinase K. A dot blot assay was carried out to assess the removal of SLP. Strains with and without SLP were exposed to 0–0.5 mM Pb(NO3)2. The maximum binding capacity (qmax) and the affinity coefficient (b) were calculated using the Langmuir equation. The structural effect of Pb2+ on microorganisms with and without SLP was determined using Raman spectroscopy. The bacterial interaction with Pb2+ led to a broadening in the phosphate bands (1,300–1,200 cm−1 region) and strong alterations on amide and carboxylate-related bands (νCOO− as and νCOO− s). Microorganisms without SLP removed higher percentages of Pb2+ and had higher qmax than those bearing SLP. Isolated SLP had much lower qmax and also removed lower percentages of Pb2+ than the corresponding whole microorganisms. The hydrofobicity of both strains dramatically dropped when removing SLP. When bearing SLP, strains do not expose a large amount of charged groups on their surfaces, thus making less efficient the Pb2+ removal. On the contrary, the extremely low hydrofobicity of microorganisms without SLP (and consequently, their higher capacity to remove Pb2+) can be explained on the basis of a greater exposure of charged chemical groups for the interaction with Pb2+. The viability of bacteria without SLP was not significantly lower than that of bacteria bearing SLP. However, microorganisms without SLP were more prone to the detrimental effect of Pb2+, thus suggesting that SLP acts as a protective rather than as a sequestrant layer.
Palabras clave: BIOSORPTION , LACTOBACILLUS KEFIR , LEAD , S-LAYER PROTEINS
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 1015.Kb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/10104
DOI: http://dx.doi.org/10.1007/s11274-015-1812-7
URL: http://link.springer.com/article/10.1007%2Fs11274-015-1812-7
Colecciones
Articulos(CIDCA)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Citación
Gerbino, Oscar Esteban; Carasi, Paula; Araujo Andrade, Cuauhtémoc; Tymczyszyn, Emma Elizabeth; Gomez Zavaglia, Andrea; Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir; Springer; World Journal of Microbiology; 31; 4; 2-2015; 583-592
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES