Artículo
Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
Svetaz, Laura Andrea
; Agüero, María Belén
; Alvarez, Sandra
; Luna, Lorena Celina
; Feresin, Gabriela Egly
; Derita, Marcos Gabriel
; Tapia, Alejandro
; Zacchino, Susana Alicia Stella
Fecha de publicación:
2007-08
Editorial:
Georg Thieme Verlag Kg
Revista:
Planta Medica
ISSN:
0032-0943
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Petroleum ether and dichloromethane extracts of fruits, aerial parts and exudate of Zuccagnia punctata Cav. (Fabaceae) showed moderate antifungal activities against the yeasts C. albicans, S. cerevisiae and C. neoformans (MICs: 62.5-250 μg/mL) and very strong antifungal activities against the dermatophytes M. gypseum, T. rubrum and T. mentagrophytes (MICs: 8-16 μg/mL) thus supporting the ethnopharmacological use of this plant. Antifungal activity-directed fractionation of active extracts by using bioautography led to the isolation of 2′,4′-dihydroxy-3′-methoxychalcone (1) and 2′,4′-dihydroxychalcone (2) as the compounds responsible for the antifungal activity. Second-order studies included MIC80, MIC 50 and MFC of both chalcones in an extended panel of clinical isolates of the most sensitive fungi, and also comprised a series of targeted assays. They showed that the most active chalcone 2 is fungicidal rather than fungistatic, does not disrupt the fungal membranes up to 4 x MFC and does not act by inhibiting the fungal cell wall. So, 2′,4′-dihydroxychalcone would act by a different mechanism of action than the antifungal drugs in current clinical use, such as amphotericin B, azoles or echinocandins, and thus appears to be very promising as a novel antifungal agent. © Georg Thieme Verlag KG Stuttgart.
Palabras clave:
ANTIFUNGAL
,
BIOAUTOGRAPHY
,
CHALCONES
,
DENSITOMETRY
,
FABACEAE
,
MECHANISM OF ACTION
,
ZUCCAGNIA PUNCTATA
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Colecciones
Articulos(CCT - NOA SUR)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos(PROBIEN)
Articulos de INST. DE INVESTIGACION Y DES. EN ING. DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Articulos de INST. DE INVESTIGACION Y DES. EN ING. DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Citación
Svetaz, Laura Andrea; Agüero, María Belén; Alvarez, Sandra; Luna, Lorena Celina; Feresin, Gabriela Egly; et al.; Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action; Georg Thieme Verlag Kg; Planta Medica; 73; 10; 8-2007; 1074-1080
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