Artículo
Nodulation and Delayed Nodule Senescence: Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
López, Silvina Marianela Yanil
; Sánchez, Ma. Dolores Molina; Pastorino, Graciela Noemí; Franco, Mario Emilio Ernesto
; García, Nicolás Toro; Balatti, Pedro Alberto
Fecha de publicación:
08/2018
Editorial:
Springer
Revista:
Current Microbiology
ISSN:
0343-8651
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The purpose of this work was to study further two Bradyrhizobium japonicum strains with high nitrogen-fixing capacity that were identified within a collection of approximately 200 isolates from the soils of Argentina. Nodulation and nitrogen-fixing capacity and the level of expression of regulatory as well as structural genes of nitrogen fixation and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene of the isolates were compared with that of E109-inoculated plants. Both isolates of B. japonicum, 163 and 366, were highly efficient to fix nitrogen compared to commercial strain E109. Isolate 366 developed a higher number and larger biomass of nodules and because of this fixed more nitrogen. Isolate 163 developed the same number and nodule biomass than E109. However, nodules developed by isolate 163 had red interiors for a longer period, had a higher leghemoglobin content, and presented high levels of expression of acdS gene, that codes for an ACC deaminase. In conclusion, naturalized rhizobia of the soils of Argentina hold a diverse population that might be the source of highly active nitrogen-fixing rhizobia, a process that appears to be based on different strategies.
Palabras clave:
Senescencia
,
Exppresion de genes
,
Bradyrhizobium
,
Soja
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
López, Silvina Marianela Yanil; Sánchez, Ma. Dolores Molina; Pastorino, Graciela Noemí; Franco, Mario Emilio Ernesto; García, Nicolás Toro; et al.; Nodulation and Delayed Nodule Senescence: Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen; Springer; Current Microbiology; 75; 8; 8-2018; 997-1005
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