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dc.contributor.author
Choi, Chan-Il
dc.contributor.author
Yoon, Sang-Phil
dc.contributor.author
Choi, Jung-Mi
dc.contributor.author
Kim, Sung-Soo
dc.contributor.author
Lee, Young-Don
dc.contributor.author
Birnbaumer, Lutz
dc.contributor.author
Suh-Kim. Haeyoung
dc.date.available
2020-03-17T13:47:16Z
dc.date.issued
2014-05-02
dc.identifier.citation
Choi, Chan-Il ; Yoon, Sang-Phil; Choi, Jung-Mi; Kim, Sung-Soo; Lee, Young-Don; et al.; Simultaneous deletion of floxed genes mediated by CaMKIIa-Cre in the brain and in male germ cells: application to conditional and conventional disruption of Go-alfa; Korean Soc Med Biochemistry Molecular Biology; Experimental And Molecular Medicine Emm; 46; 2-5-2014; e93
dc.identifier.issn
1226-3613
dc.identifier.uri
http://hdl.handle.net/11336/99772
dc.description.abstract
The Cre/LoxP system is a well-established approach to spatially and temporally control genetic inactivation. The calcium/calmodulin-dependent protein kinase II alpha subunit (CaMKIIα) promoter limits expression to specific regions of the forebrain and thus has been utilized for the brain-specific inactivation of the genes. Here, we show that CaMKIIα-Cre can be utilized for simultaneous inactivation of genes in the adult brain and in male germ cells. Double transgenic Rosa26+/stop-lacZ::CaMKIIα-Cre+/Cre mice generated by crossing CaMKIIα-Cre+/Cre mice with floxed ROSA26 lacZ reporter (Rosa26+/stop-lacZ) mice exhibited lacZ expression in the brain and testis. When these mice were mated to wild-type females, about 27% of the offspring were whole body blue by X-gal staining without inheriting the Cre transgene. These results indicate that recombination can occur in the germ cells of male Rosa26+/stop-lacZ::CaMKIIα-Cre+/Cre mice. Similarly, when double transgenic Gnao+/f::CaMKIIα-Cre+/Cre mice carrying a floxed Go-alpha gene (Gnaof/f) were backcrossed to wild-type females, approximately 22% of the offspring carried the disrupted allele (GnaoΔ) without inheriting the Cre transgene. The GnaoΔ/Δ mice closely resembled conventional Go-alpha knockout mice (Gnao−/−) with respect to impairment of their behavior. Thus, we conclude that CaMKIIα-Cre mice afford recombination for both tissue- and time-controlled inactivation of floxed target genes in the brain and for their permanent disruption. This work also emphasizes that extra caution should be exercised in utilizing CaMKIIα-Cre mice as breeding pairs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Korean Soc Med Biochemistry Molecular Biology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BRAIN
dc.subject
CRE
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CaMKII ALPHA
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GNAO
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TESTIS
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Simultaneous deletion of floxed genes mediated by CaMKIIa-Cre in the brain and in male germ cells: application to conditional and conventional disruption of Go-alfa
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-28T18:26:31Z
dc.journal.volume
46
dc.journal.pagination
e93
dc.journal.pais
Corea del Sur
dc.description.fil
Fil: Choi, Chan-Il. Ajou University. School of Medicine; Corea del Sur
dc.description.fil
Fil: Yoon, Sang-Phil. Ajou University. School of Medicine; Corea del Sur
dc.description.fil
Fil: Choi, Jung-Mi. Ajou University. School of Medicine; Corea del Sur
dc.description.fil
Fil: Kim, Sung-Soo. Ajou University. School of Medicine; Corea del Sur
dc.description.fil
Fil: Lee, Young-Don. Ajou University. School of Medicine; Corea del Sur
dc.description.fil
Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences; Estados Unidos. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil
Fil: Suh-Kim. Haeyoung. Ajou University. School of Medicine; Corea del Sur
dc.journal.title
Experimental And Molecular Medicine Emm
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/emm201414
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/emm.2014.14
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