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dc.contributor.author
Cotabarren, Juliana  
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Tellechea, Mariana Edith  
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Tanco, Sebastián Martín  
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Lorenzo Rivera, Julia  
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Garcia Pardo, Javier  
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Avilés, Francesc Xavier  
dc.contributor.author
Obregon, Walter David  
dc.date.available
2020-03-16T20:22:49Z  
dc.date.issued
2018-03  
dc.identifier.citation
Cotabarren, Juliana; Tellechea, Mariana Edith; Tanco, Sebastián Martín; Lorenzo Rivera, Julia; Garcia Pardo, Javier; et al.; Biochemical and MALDI-TOF mass spectrometric characterization of a novel native and recombinant cystine knot miniprotein from Solanum tuberosum subsp. andigenum cv. Churqueña; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 19; 3; 3-2018  
dc.identifier.issn
1422-0067  
dc.identifier.uri
http://hdl.handle.net/11336/99693  
dc.description.abstract
Cystine-knot miniproteins (CKMPs) are an intriguing group of cysteine-rich molecules that combine the characteristics of proteins and peptides. Typically, CKMPs are fewer than 50 residues in length and share a characteristic knotted scaffold characterized by the presence of three intramolecular disulfide bonds that form the singular knotted structure. The knot scaffold confers on these proteins remarkable chemical, thermal, and proteolytic stability. Recently, CKMPs have emerged as a novel class of natural molecules with interesting pharmacological properties. In the present work, a novel cystine-knot metallocarboxypeptidase inhibitor (chuPCI) was isolated from tubers of Solanum tuberosum, subsp. andigenum cv. Churqueña. Our results demonstrated that chuPCI is a member of the A/B-type family of metallocarboxypeptidases inhibitors. chuPCI was expressed and characterized by a combination of biochemical and mass spectrometric techniques. Direct comparison of the MALDI-TOF mass spectra for the native and recombinant molecules allowed us to confirm the presence of four different forms of chuPCI in the tubers. The majority of such forms have a molecular weight of 4309 Da and contain a cyclized Gln in the N-terminus. The other three forms are derived from N-terminal and/or C-terminal proteolytic cleavages. Taken together, our results contribute to increase the current repertoire of natural CKMPs.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Molecular Diversity Preservation International  
dc.rights
info:eu-repo/semantics/openAccess  
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
ANDEAN POTATOES  
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CARBOXYPEPTIDASE INHIBITOR  
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CYSTINE-KNOT MINIPROTEINS  
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PLANT INHIBITOR  
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PROTEASE  
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SOLANUM TUBEROSUM  
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Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Biochemical and MALDI-TOF mass spectrometric characterization of a novel native and recombinant cystine knot miniprotein from Solanum tuberosum subsp. andigenum cv. Churqueña  
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info:eu-repo/semantics/article  
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info:ar-repo/semantics/artículo  
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info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-03-12T18:43:05Z  
dc.journal.volume
19  
dc.journal.number
3  
dc.journal.pais
Suiza  
dc.journal.ciudad
Basel  
dc.description.fil
Fil: Cotabarren, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina  
dc.description.fil
Fil: Tellechea, Mariana Edith. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Universitat Autònoma de Barcelona; España  
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Fil: Tanco, Sebastián Martín. Universitat Autònoma de Barcelona; España  
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Fil: Lorenzo Rivera, Julia. Universitat Autònoma de Barcelona; España  
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Fil: Garcia Pardo, Javier. Universitat Autònoma de Barcelona; España  
dc.description.fil
Fil: Avilés, Francesc Xavier. Universitat Autònoma de Barcelona; España  
dc.description.fil
Fil: Obregon, Walter David. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
International Journal of Molecular Sciences  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1422-0067/19/3/678  
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info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.3390/ijms19030678