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dc.contributor.author
Marinangeli, Pablo Alejandro
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dc.contributor.other
Germaná, María Antonietta
dc.contributor.other
Lambardi, Maurizio
dc.date.available
2020-03-16T18:38:50Z
dc.date.issued
2016
dc.identifier.citation
Marinangeli, Pablo Alejandro; Somatic Embryogenesis of Lilium from Microbulb Transverse Thin Cell Layers; Humana Press; 1359; 2016; 331-340
dc.identifier.isbn
9781493930609
dc.identifier.uri
http://hdl.handle.net/11336/99668
dc.description.abstract
A reliable somatic embryogenesis protocol is a prerequisite for application of other plant biotechniques.Several protocols were reported for genus Lilium, with variable success. Between them, transverse ThinCell Layers (tTCL) were used efficiently to induce indirect somatic embryogenesis of Lilium. Somaticembryogenesis potential is dependent on the genotype, explant, and culture medium composition,especially as for plant growth regulators and environmental conditions. Usually, the process comprisesthree phases: embryogenic callus induction, embryogenic callus proliferation and somatic embryogermination. Somatic embryo germination can be achieved in light or dark. In the first case, completeplantlets are formed, with green leaves and pseudobulb in the base. In darkness, microbulbs are formedfrom single somatic embryos or clusters. A last phase of microbulb enlargement allows plantlets ormicrobulbs to increase their biomass. These enlarged microbulbs do not need special acclimatizationconditions when transferred to soil and quickly produce sturdy plants. This chapter describes a protocolfor somatic embryogenesis of Lilium using tTCL from microbulbs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
EMBRIOGENESIS SOMATICA
dc.subject
LILIUM
dc.subject
THIN CELL LAYERS
dc.subject
IN VITRO CULTURE
dc.subject.classification
Biotecnología Agrícola y Biotecnología Alimentaria
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dc.subject.classification
Biotecnología Agropecuaria
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Somatic Embryogenesis of Lilium from Microbulb Transverse Thin Cell Layers
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/bookPart
dc.type
info:ar-repo/semantics/parte de libro
dc.date.updated
2020-02-19T18:58:57Z
dc.journal.volume
1359
dc.journal.pagination
331-340
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
New York
dc.description.fil
Fil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-4939-3061-6_19
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/978-1-4939-3061-6_19
dc.conicet.paginas
558
dc.source.titulo
In Vitro Embryogenesis in Higher Plants
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