Artículo
M1 Macrophage Polarization Is Dependent on TRPC1-Mediated Calcium Entry
Chauhan, Arun; Sun, Yuyang; Sukumaran, Pramod; Quenum Zangbede, Fredice O.; Jondle, Christopher N.; Sharma, Atul; Evans, Dustin L.; Chauhan, Pooja; Szlabick, Randolph E.; Aaland, Mary O.; Birnbaumer, Lutz
; Sharma, Jyotika; Singh, Brij B.; Mishra, Bibhuti B.
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
10/2018
Editorial:
Elsevier Inc.
Revista:
iScience
ISSN:
2589-0042
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Macrophage plasticity is essential for innate immunity, but in-depth signaling mechanism(s) regulating their functional phenotypes are ill-defined. Here we report that interferon (IFN) g priming of naive macrophages induces store-mediated Ca2+ entry and inhibition of Ca2+ entry impairs polarization to M1 inflammatory phenotype. In vitro and in vivo functional analyses revealed ORAI1 to be a primary contributor to basal Ca2+ influx in macrophages, whereas IFNg-induced Ca2+ influx was mediated by TRPC1. Deficiency of TRPC1 displayed abrogated IFNg-induced M1 inflammatory mediators in macrophages. In a preclinical model of peritonitis by Klebsiella pneumoniae infection, macrophages showed increased Ca2+ influx, which was TRPC1 dependent. Macrophages from infected TRPC1 / mice showed inhibited expression of M1-associated signature molecules. Furthermore, in human patients with systemic inflammatory response syndrome, the level of TRPC1 expression in circulating macrophages directly correlated with M1 inflammatory mediators. Overall, TRPC1-mediated Ca2+ influx is essential for the induction/shaping of macrophage polarization to M1 inflammatory phenotype.
Palabras clave:
BIOLOGICAL SCIENCES
,
IMMUNE RESPONSE
,
IMMUNOLOGY
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(BIOMED)
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Citación
Chauhan, Arun; Sun, Yuyang; Sukumaran, Pramod; Quenum Zangbede, Fredice O.; Jondle, Christopher N.; et al.; M1 Macrophage Polarization Is Dependent on TRPC1-Mediated Calcium Entry; Elsevier Inc.; iScience; 8; 10-2018; 85-102
Compartir
Altmétricas