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dc.contributor.author
Levi, Valeria  
dc.contributor.author
Serpinskaya, Anna S.  
dc.contributor.author
Gratton, Enrico  
dc.contributor.author
Gelfand, Vladimir  
dc.date.available
2020-03-12T20:43:28Z  
dc.date.issued
2006-12  
dc.identifier.citation
Levi, Valeria; Serpinskaya, Anna S.; Gratton, Enrico; Gelfand, Vladimir; Organelle transport along microtubules in Xenopus melanophores: Evidence for cooperation between multiple motors; Elsevier; Biophysical Journal; 90; 1; 12-2006; 318-327  
dc.identifier.issn
0006-3495  
dc.identifier.uri
http://hdl.handle.net/11336/99387  
dc.description.abstract
Xenopus melanophores have pigment organelles or melanosomes which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. Melanosomes are transported by microtubule motors, kinesin-2 and cytoplasmic dynein, and an actin motor, myosin-V. We explored the regulation of melanosome transport along microtubules in vivo by using a new fast-tracking routine, which determines the melanosome position every 10 ms with 2-nm precision. The velocity distribution of melanosomes transported by cytoplasmic dynein or kinesin-2 under conditions of aggregation and dispersion presented several peaks and could not be fit with a single Gaussian function. We postulated that the melanosome velocity depends linearly on the number of active motors. According to this model, one to three dynein molecules transport each melanosome in the minus-end direction. The transport in the plus-end direction is mainly driven by one to two copies of kinesin-2. The number of dyneins transporting a melanosome increases during aggregation, whereas the number of active kinesin-2 stays the same during aggregation and dispersion. Thus, the number of active dynein molecules regulates the net direction of melanosome transport. The model also shows that multiple motors of the same polarity cooperate during the melanosome transport, whereas motors of opposite polarity do not compete.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
organelle  
dc.subject.classification
Biofísica  
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Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Organelle transport along microtubules in Xenopus melanophores: Evidence for cooperation between multiple motors  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-02-13T20:09:30Z  
dc.journal.volume
90  
dc.journal.number
1  
dc.journal.pagination
318-327  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Ámsterdam  
dc.description.fil
Fil: Levi, Valeria. University of Illinois at Urbana; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina  
dc.description.fil
Fil: Serpinskaya, Anna S.. Northwestern University; Estados Unidos  
dc.description.fil
Fil: Gratton, Enrico. University of Illinois at Urbana; Estados Unidos  
dc.description.fil
Fil: Gelfand, Vladimir. Northwestern University; Estados Unidos  
dc.journal.title
Biophysical Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006349506722135  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1529/biophysj.105.067843