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dc.contributor.author
Consentino, Laurent
dc.contributor.author
Lambert, Stefan
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Martino, Carlos
dc.contributor.author
Jourdan, Nathalie
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Bouchet, Pierre Etienne
dc.contributor.author
Witczak, Jacques
dc.contributor.author
Castello, Pablo Raul
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dc.contributor.author
El-Esawi, Mohamed
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Corbineau, Francoise
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d'Harlingue, Alain
dc.contributor.author
Ahmad, Margaret
dc.date.available
2020-03-12T17:56:17Z
dc.date.issued
2015-06
dc.identifier.citation
Consentino, Laurent; Lambert, Stefan; Martino, Carlos; Jourdan, Nathalie; Bouchet, Pierre Etienne; et al.; Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism; Wiley Blackwell Publishing, Inc; New Phytologist; 206; 4; 6-2015; 1450-1462
dc.identifier.issn
0028-646X
dc.identifier.uri
http://hdl.handle.net/11336/99292
dc.description.abstract
Cryptochromes are widespread blue-light absorbing flavoproteins with important signaling roles. In plants they mediate de-etiolation, developmental and stress responses resulting from interaction with downstream signaling partners such as transcription factors and components of the proteasome. Recently, it has been shown that Arabidopsis cry1 activation by blue light also results in direct enzymatic conversion of molecular oxygen (O2) to reactive oxygen species (ROS) and hydrogen peroxide (H2O2) in vitro. Here we explored whether direct enzymatic synthesis of ROS by Arabidopsis cry1 can play a physiological role in vivo. ROS formation resulting from cry1 expression was measured by fluorescence assay in insect cell cultures and in Arabidopsis protoplasts from cryptochrome mutant seedlings. Cell death was determined by colorimetric assay. We found that ROS formation results from cry1 activation and induces cell death in insect cell cultures. In plant protoplasts, cryptochrome activation results in rapid increase in ROS formation and cell death. We conclude that ROS formation by cryptochromes may indeed be of physiological relevance and could represent a novel paradigm for cryptochrome signaling.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ARABIDOPSIS THALIANA
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CRYPTOCHROME
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OXIDATIVE STRESS
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PHOTOMORPHOGENESIS
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PHOTORECEPTOR
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REACTIVE OXYGEN SPECIES (ROS) SIGNALING
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-02-26T15:05:29Z
dc.journal.volume
206
dc.journal.number
4
dc.journal.pagination
1450-1462
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Consentino, Laurent. Université Pierre et Marie Curie; Francia
dc.description.fil
Fil: Lambert, Stefan. Université Pierre et Marie Curie; Francia
dc.description.fil
Fil: Martino, Carlos. Université Pierre et Marie Curie; Francia. University of Florida; Estados Unidos
dc.description.fil
Fil: Jourdan, Nathalie. Université Pierre et Marie Curie; Francia
dc.description.fil
Fil: Bouchet, Pierre Etienne. Université Pierre et Marie Curie; Francia
dc.description.fil
Fil: Witczak, Jacques. Université Pierre et Marie Curie; Francia
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Fil: Castello, Pablo Raul. Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Université Pierre et Marie Curie; Francia
dc.description.fil
Fil: El-Esawi, Mohamed. Université Pierre et Marie Curie; Francia. Tanta University; Egipto
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Fil: Corbineau, Francoise. Université Pierre et Marie Curie; Francia
dc.description.fil
Fil: d'Harlingue, Alain. Université Pierre et Marie Curie; Francia
dc.description.fil
Fil: Ahmad, Margaret. Université Pierre et Marie Curie; Francia. Universidad Xavier; Estados Unidos
dc.journal.title
New Phytologist
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.13341
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/nph.13341
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