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dc.contributor.author
Vissani, María Aldana
dc.contributor.author
Tordoya, Maria Silvia
dc.contributor.author
Tsai, Y.-L.
dc.contributor.author
Lee, P.-Y.A.
dc.contributor.author
Shen, Y.-H.
dc.contributor.author
Lee, F.-C.
dc.contributor.author
Wang, H.-T.T.
dc.contributor.author
Parreño, Gladys Viviana
dc.contributor.author
Barrandeguy, María Edith
dc.date.available
2020-03-11T22:02:41Z
dc.date.issued
2018-07
dc.identifier.citation
Vissani, María Aldana; Tordoya, Maria Silvia; Tsai, Y.-L.; Lee, P.-Y.A.; Shen, Y.-H.; et al.; On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions; Elsevier Science; Journal of Virological Methods; 257; 7-2018; 29-32
dc.identifier.issn
0166-0934
dc.identifier.uri
http://hdl.handle.net/11336/99245
dc.description.abstract
Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ECE
dc.subject
EQUID HERPESVIRUS 3
dc.subject
IIPCR
dc.subject
ON-SITE DETECTION
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-03-11T13:03:09Z
dc.journal.volume
257
dc.journal.pagination
29-32
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Vissani, María Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Tsai, Y.-L.. GeneReach; Estados Unidos
dc.description.fil
Fil: Lee, P.-Y.A.. GeneReach; Estados Unidos
dc.description.fil
Fil: Shen, Y.-H.. GeneReach; Estados Unidos
dc.description.fil
Fil: Lee, F.-C.. GeneReach; Estados Unidos
dc.description.fil
Fil: Wang, H.-T.T.. GeneReach; Estados Unidos
dc.description.fil
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Barrandeguy, María Edith. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
dc.journal.title
Journal of Virological Methods
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166093418300260
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jviromet.2018.04.002
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