Artículo
An enzymatic alternative for the synthesis of nucleoside 5′-monophosphates
Fecha de publicación:
04/2018
Editorial:
Elsevier Science Inc
Revista:
Enzyme and Microbial Technology
ISSN:
0141-0229
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A new procedure was carried out for the synthesis of nucleoside 5′-monophosphates, involving the use of two enzymes. The first step applied phospholipase D from Streptomyces netropsis and phosphatidylcholine as phosphatidyl donor, to give 5′-(3-sn-phosphatidyl) nucleosides (C, U, A, I). These were selectively hydrolysed in the second step by the action of phospholipase C from Bacillus cereus to produce the respective 5′-nucleotides. Application of this methodology on a preparative scale conducted to 5′-adenosine monophosphate in 63% overall yield from adenosine. The regioselectivity of these enzymes avoids protection steps, the overall synthesis is performed under mild reaction conditions and product isolation is easily achieved.
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Articulos(SEDE CENTRAL)
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Articulos de SEDE CENTRAL
Citación
Gudiño, Esteban Dario; Santillan, Julia Yamila; Iglesias, Luis Emilio; Iribarren, Adolfo Marcelo; An enzymatic alternative for the synthesis of nucleoside 5′-monophosphates; Elsevier Science Inc; Enzyme and Microbial Technology; 111; 4-2018; 1-6
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